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PMID: 6325157 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and characterization of the chicken DNA locus related to the oncogene erbB of avian erythroblastosis virus.

The EMBO journal ·Vol. 1 ·No. 2 ·1982-00-00 ·Pages 237-42

Sergeant A, Saule S, Leprince D, Begue A, Rommens C, Stehelin D

Abstract

Chicken cell DNA contains sequences which are homologous to the avian erythroblastosis virus oncogene v-erb. These cellular sequences (c-erb) have been isolated from a library of chicken cell DNA fragments generated by partial digestion with AluI and HaeIII and shown to be shared by at least two loci in the chicken DNA. One of them, denoted c-erbB, contains approximately 1.8 kilobase pairs of chicken DNA homologous to the 3' part of the v-erb oncogene (v-erbB). Restriction mapping studies show that the c-erbB DNA sequences homologous to v-erbB are distributed among six EcoRI fragments located in a single genomic region. Heteroduplexes between v-erbB in viral RNA and cloned c-erbB DNA show that the chicken DNA sequences homologous to v-erbB are interrupted by 11 DNA sequences not present in the v-erb oncogene. We conclude from our data that the c-erbB locus might represent the cellular progenitor for the v-erbB domain of the v-erb oncogene.

MeSH Terms
Alpharetrovirus/genetics Animals Avian Leukosis Virus/genetics Chickens Cloning, Molecular DNA/analysis DNA Restriction Enzymes/metabolism DNA, Viral/analysis Microscopy, Electron Nucleic Acid Hybridization Oncogenes
Chemicals
DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sergeant A
Saule S
Leprince D
Begue A
Rommens C
Stehelin D
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
237-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553026
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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