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PMID: 6246260 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell-free translation of avian erythroblastosis virus RNA.

Journal of virology ·Vol. 34 ·No. 1 ·1980-04-00 ·Pages 280-4

Pawson T, Martin GS

Abstract

Avian erythroblastosis virus (AEV) RNA rescued from nonproducer cells by superinfection with a helper virus is translated into three polypeptides in the messenger-dependent rabbit reticulocyte lysate. A 75,000 molecular weight polypeptide (P75AEV) is synthesized from 28S RNA and is encoded by the 5' section of the AEV RNA, including gag-related and AEV-specific sequences. The P75AEV synthesized in infected cells and the P75AEV synthesized in the cell-free system are electrophoretically identical. A 44,000 molecular weight polypeptide (P44AEV) is synthesized from 20-24S RNA, apparently from the 3' section of the AEV-specific RNA sequence. A minor 37,000 molecular weight polypeptide (P37AEV) is synthesized from 20S AEV RNA. A comparison is drawn between the cell-free products of MC29 and AEV RNAs.

MeSH Terms
Alpharetrovirus/genetics Avian Leukosis Virus/genetics Cell-Free System Genes, Viral Molecular Weight Protein Biosynthesis RNA, Viral/genetics Viral Proteins/analysis,biosynthesis,genetics
Chemicals
RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pawson T
Martin G S
References (18)
18 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.1.280-284.1980
Published
1980-04-00
Pages
280-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288696
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.34.1.280-284.1980 PMC Full Text View journal details
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