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PMID: 16219777 Published · ppublish English Comparative Study Journal Article

Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage.

Genetics ·Vol. 172 ·No. 1 ·2006-01-00 ·Pages 67-76

Redon C, Pilch DR, Bonner WM

Abstract

Collision between a topoisomerase I-DNA intermediate and an advancing replication fork represents a unique form of replicative damage. We have shown previously that yeast H2A serine 129 is involved in the recovery from this type of damage. We now report that efficient repair also requires proteins involved in chromatid cohesion: Csm3; Tof1; Mrc1, and Dcc1. Epistasis analysis defined several pathways involving these proteins. Csm3 and Tof1 function in a same pathway and downstream of H2A. In addition, the pathway involving H2A/Csm3/Tof1 is distinct from the pathways involving the Ctf8/Ctf18/Dcc1 complex, the Rad9 pathway, and another involving Mrc1. Our genetic studies suggest a role for H2A serine 129 in the establishment of specialized cohesion structure necessary for the normal repair of topoisomerase I-induced DNA damage.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism Chromatids/metabolism Chromosomal Proteins, Non-Histone/genetics,metabolism DNA Damage DNA Repair DNA Replication DNA Topoisomerases, Type I/metabolism DNA-Binding Proteins/genetics,metabolism Histones/genetics,metabolism Mutation/genetics Rad52 DNA Repair and Recombination Protein/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Serine/chemistry,genetics
Chemicals
CTF18 protein, S cerevisiae Cell Cycle Proteins Chromosomal Proteins, Non-Histone Ctf8 protein, S cerevisiae DNA-Binding Proteins Dcc1 protein, S cerevisiae Histones MRC1 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins TOF1 protein, S cerevisiae Serine DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Redon Christophe
NIH, NCI, DBS, Laboratory of Molecular Pharmacology, Bethesda, Maryland 20892, USA.
Pilch Duane R
Bonner William M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.105.046128
Published
2006-01-00
Epub
2005-00-11
Pages
67-76
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1456192
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.105.046128 PMC Full Text View journal details
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