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PMID: 11486023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway.

Molecular and cellular biology ·Vol. 21 ·No. 17 ·2001-09-00 ·Pages 5838-45

Naiki T, Kondo T, Nakada D, Matsumoto K, Sugimoto K

Abstract

RAD24 has been identified as a gene essential for the DNA damage checkpoint in budding yeast. Rad24 is structurally related to subunits of the replication factor C (RFC) complex, and forms an RFC-related complex with Rfc2, Rfc3, Rfc4, and Rfc5. The rad24Delta mutation enhances the defect of rfc5-1 in the DNA replication block checkpoint, implicating RAD24 in this checkpoint. CHL12 (also called CTF18) encodes a protein that is structurally related to the Rad24 and RFC proteins. We show here that although neither chl12Delta nor rad24Delta single mutants are defective, chl12Delta rad24Delta double mutants become defective in the replication block checkpoint. We also show that Chl12 interacts physically with Rfc2, Rfc3, Rfc4, and Rfc5 and forms an RFC-related complex which is distinct from the RFC and RAD24 complexes. Our results suggest that Chl12 forms a novel RFC-related complex and functions redundantly with Rad24 in the DNA replication block checkpoint.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism DNA Damage DNA Replication DNA-Binding Proteins/metabolism Fungal Proteins/genetics,metabolism Intracellular Signaling Peptides and Proteins Replication Protein C Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Signal Transduction
Chemicals
CTF18 protein, S cerevisiae Cell Cycle Proteins DNA-Binding Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins RAD24 protein, S cerevisiae RFC2 protein, S cerevisiae RFC4 protein, human Saccharomyces cerevisiae Proteins Replication Protein C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Naiki T
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-0814, Japan.
Kondo T
Nakada D
Matsumoto K
Sugimoto K
References (33)
33 references, click to expand
  1. A sliding clamp model for the Rad1 family of cell cycle checkpoint proteins.
    Cell. 1999 Mar 19;96(6):769-70 PMID: 10102265
  2. G2/M checkpoint genes of Saccharomyces cerevisiae: further evidence for roles in DNA replication and/or repair.
    Mol Gen Genet. 1997 Nov;256(6):638-51 PMID: 9435789
  3. The yeast Sgs1p helicase acts upstream of Rad53p in the DNA replication checkpoint and colocalizes with Rad53p in S-phase-specific foci.
    Genes Dev. 2000 Jan 1;14(1):81-96 PMID: 10640278
  4. A novel Rad24 checkpoint protein complex closely related to replication factor C.
    Curr Biol. 2000 Jan 13;10(1):39-42 PMID: 10660302
  5. Structure-based predictions of Rad1, Rad9, Hus1 and Rad17 participation in sliding clamp and clamp-loading complexes.
    Nucleic Acids Res. 2000 Jul 1;28(13):2481-93 PMID: 10871397
  6. Rfc5, in cooperation with rad24, controls DNA damage checkpoints throughout the cell cycle in Saccharomyces cerevisiae.
    Mol Cell Biol. 2000 Aug;20(16):5888-96 PMID: 10913172
  7. The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast.
    Genes Dev. 2000 Aug 15;14(16):2046-59 PMID: 10950868
  8. LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae.
    EMBO J. 2000 Nov 1;19(21):5801-12 PMID: 11060031
  9. The DNA damage response: putting checkpoints in perspective.
    Nature. 2000 Nov 23;408(6811):433-9 PMID: 11100718
  10. Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae.
    Mol Cell Biol. 2001 Feb;21(3):755-64 PMID: 11154263
  11. A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.
    Nat Cell Biol. 1999 Nov;1(7):393-8 PMID: 10559981
  12. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  13. Checkpoints: controls that ensure the order of cell cycle events.
    Science. 1989 Nov 3;246(4930):629-34 PMID: 2683079
  14. CHL12, a gene essential for the fidelity of chromosome transmission in the yeast Saccharomyces cerevisiae.
    Genetics. 1994 Dec;138(4):1067-79 PMID: 7896091
  15. Characterization of the five replication factor C genes of Saccharomyces cerevisiae.
    Mol Cell Biol. 1995 Sep;15(9):4661-71 PMID: 7651383
  16. Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins.
    EMBO J. 1995 Dec 1;14(23):5812-23 PMID: 8846774
  17. Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways.
    Science. 1996 Jan 19;271(5247):357-60 PMID: 8553072
  18. Spk1/Rad53 is regulated by Mec1-dependent protein phosphorylation in DNA replication and damage checkpoint pathways.
    Genes Dev. 1996 Feb 15;10(4):395-406 PMID: 8600024
  19. Rfc5, a small subunit of replication factor C complex, couples DNA replication and mitosis in budding yeast.
    Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7048-52 PMID: 8692942
  20. Cell cycle checkpoints: preventing an identity crisis.
    Science. 1996 Dec 6;274(5293):1664-72 PMID: 8939848
  21. RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage.
    Genetics. 1997 Jan;145(1):45-62 PMID: 9017389
  22. A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme.
    J Biol Chem. 1997 Jul 25;272(30):18974-81 PMID: 9228079
  23. Rfc5, a replication factor C component, is required for regulation of Rad53 protein kinase in the yeast checkpoint pathway.
    Mol Cell Biol. 1997 Oct;17(10):5905-14 PMID: 9315648
  24. Rad53-dependent phosphorylation of Swi6 and down-regulation of CLN1 and CLN2 transcription occur in response to DNA damage in Saccharomyces cerevisiae.
    Genes Dev. 1997 Nov 15;11(22):3032-45 PMID: 9367985
  25. Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint.
    Science. 1998 Jul 10;281(5374):272-4 PMID: 9657725
  26. Functional and physical interaction between Rad24 and Rfc5 in the yeast checkpoint pathways.
    Mol Cell Biol. 1998 Sep;18(9):5485-91 PMID: 9710632
  27. MEC1-dependent phosphorylation of Rad9p in response to DNA damage.
    Mol Cell. 1998 Aug;2(2):183-9 PMID: 9734355
  28. DNA damage checkpoint in budding yeast.
    EMBO J. 1998 Oct 1;17(19):5525-8 PMID: 9755152
  29. The budding yeast Rad9 checkpoint protein is subjected to Mec1/Tel1-dependent hyperphosphorylation and interacts with Rad53 after DNA damage.
    EMBO J. 1998 Oct 1;17(19):5679-88 PMID: 9755168
  30. The DNA replication fork in eukaryotic cells.
    Annu Rev Biochem. 1998;67:721-51 PMID: 9759502
  31. Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway.
    Mol Cell Biol. 1999 Feb;19(2):1136-43 PMID: 9891048
  32. Conserved domains in DNA repair proteins and evolution of repair systems.
    Nucleic Acids Res. 1999 Mar 1;27(5):1223-42 PMID: 9973609
  33. Replication factor C3 of Schizosaccharomyces pombe, a small subunit of replication factor C complex, plays a role in both replication and damage checkpoints.
    Mol Biol Cell. 1999 Dec;10(12):3991-4003 PMID: 10588638
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.17.5838-5845.2001
Published
2001-09-00
Pages
5838-45
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87303
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.21.17.5838-5845.2001 PMC Full Text View journal details
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