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PMID: 16207866 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Role of GABAergic inhibition in shaping odor-evoked spatiotemporal patterns in the Drosophila antennal lobe.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 25 ·No. 40 ·2005-10-05 ·Pages 9069-79

Wilson RI, Laurent G

Abstract

Drosophila olfactory receptor neurons project to the antennal lobe, the insect analog of the mammalian olfactory bulb. GABAergic synaptic inhibition is thought to play a critical role in olfactory processing in the antennal lobe and olfactory bulb. However, the properties of GABAergic neurons and the cellular effects of GABA have not been described in Drosophila, an important model organism for olfaction research. We have used whole-cell patch-clamp recording, pharmacology, immunohistochemistry, and genetic markers to investigate how GABAergic inhibition affects olfactory processing in the Drosophila antennal lobe. We show that many axonless local neurons (LNs) in the adult antennal lobe are GABAergic. GABA hyperpolarizes antennal lobe projection neurons (PNs) via two distinct conductances, blocked by a GABAA- and GABAB-type antagonist, respectively. Whereas GABAA receptors shape PN odor responses during the early phase of odor responses, GABAB receptors mediate odor-evoked inhibition on longer time scales. The patterns of odor-evoked GABAB-mediated inhibition differ across glomeruli and across odors. Finally, we show that LNs display broad but diverse morphologies and odor preferences, suggesting a cellular basis for odor- and glomerulus-dependent patterns of inhibition. Together, these results are consistent with a model in which odors elicit stimulus-specific spatial patterns of GABA release, and as a result, GABAergic inhibition increases the degree of difference between the neural representations of different odors.

MeSH Terms
Animals Animals, Genetically Modified CD8 Antigens/biosynthesis Cell Count Drosophila Drosophila Proteins/metabolism Fushi Tarazu Transcription Factors/metabolism Green Fluorescent Proteins/biosynthesis Immunohistochemistry/methods Membrane Potentials/physiology Neural Inhibition/physiology Odorants Olfactory Bulb/cytology,drug effects Olfactory Receptor Neurons/drug effects,physiology Organophosphorus Compounds/pharmacology Patch-Clamp Techniques/methods Recombinant Fusion Proteins/metabolism Sense Organs/cytology,physiology Synaptic Transmission/drug effects,physiology gamma-Aminobutyric Acid/metabolism
Chemicals
CD8 Antigens Drosophila Proteins Fushi Tarazu Transcription Factors GAL4-ftzQ protein, Drosophila Organophosphorus Compounds Recombinant Fusion Proteins Green Fluorescent Proteins CGP 54626 gamma-Aminobutyric Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson Rachel I
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA. rachel_wilson@hms.harvard.edu
Laurent Gilles
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.2070-05.2005
Email
rachel_wilson@hms.harvard.edu
Published
2005-10-05
Pages
9069-79
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6725763
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.2070-05.2005 PMC Full Text View journal details
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