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PMID: 8016117 Published · ppublish English Comparative Study Journal Article

Cloning and functional expression of a Drosophila gamma-aminobutyric acid receptor.

Chen R, Belelli D, Lambert JJ, Peters JA, Reyes A, Lan NC

Abstract

A cDNA encoding a functional gamma-aminobutyric (GABA)-activated Cl- channel has been isolated from an adult Drosophila head cDNA library. When expressed in Xenopus laevis oocytes, the subunit functions efficiently, presumably as a homooligomeric complex and is activated by GABA or muscimol. GABA-evoked currents are highly sensitive to antagonism by picrotoxin but are insensitive to bicuculline, RU 5135, or zinc. Pentobarbitone greatly enhances GABA-evoked currents, whereas the neurosteroid 5 alpha-pregnan-3 alpha-ol-20-one demonstrates a large reduction in both the potency and maximal effect when compared with its actions upon vertebrate GABA type A receptors. Although zinc-insensitive, the subunit is also insensitive to flunitrazepam. Hence, the GABA receptors formed by this subunit exhibit a unique pharmacology when compared with vertebrate GABA type A receptors or those composed of rho subunits. Because the receptor-channel complex functions as a homooligomer, this subunit may be of value in mutagenesis studies aiming to define drug-binding sites.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Bicuculline/pharmacology Bridged Bicyclo Compounds/pharmacology Bridged Bicyclo Compounds, Heterocyclic Cloning, Molecular Convulsants/pharmacology DNA Primers DNA, Complementary/analysis Drosophila melanogaster/genetics,metabolism Female Flunitrazepam/pharmacology Gene Expression Macromolecular Substances Molecular Sequence Data Muscimol/pharmacology Oocytes/drug effects,physiology Pentobarbital/pharmacology Picrotoxin/pharmacology Polymerase Chain Reaction Receptors, GABA/biosynthesis,physiology Receptors, GABA-A/physiology Transcription, Genetic Xenopus laevis gamma-Aminobutyric Acid/pharmacology
Chemicals
Bridged Bicyclo Compounds Bridged Bicyclo Compounds, Heterocyclic Convulsants DNA Primers DNA, Complementary Macromolecular Substances Receptors, GABA Receptors, GABA-A Picrotoxin Muscimol gamma-Aminobutyric Acid Flunitrazepam tert-butylbicyclophosphorothionate Pentobarbital Bicuculline
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chen R
CoCensys, Inc., Irvine, CA 92718.
Belelli D
Lambert J J
Peters J A
Reyes A
Lan N C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1994-06-21
Pages
6069-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44139
Subset
IM
Databases
GENBANK
U02042
Corrections
ErratumIn
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