Abstract
A cDNA encoding a functional gamma-aminobutyric (GABA)-activated Cl- channel has been isolated from an adult Drosophila head cDNA library. When expressed in Xenopus laevis oocytes, the subunit functions efficiently, presumably as a homooligomeric complex and is activated by GABA or muscimol. GABA-evoked currents are highly sensitive to antagonism by picrotoxin but are insensitive to bicuculline, RU 5135, or zinc. Pentobarbitone greatly enhances GABA-evoked currents, whereas the neurosteroid 5 alpha-pregnan-3 alpha-ol-20-one demonstrates a large reduction in both the potency and maximal effect when compared with its actions upon vertebrate GABA type A receptors. Although zinc-insensitive, the subunit is also insensitive to flunitrazepam. Hence, the GABA receptors formed by this subunit exhibit a unique pharmacology when compared with vertebrate GABA type A receptors or those composed of rho subunits. Because the receptor-channel complex functions as a homooligomer, this subunit may be of value in mutagenesis studies aiming to define drug-binding sites.
MeSH Terms
Amino Acid Sequence
Animals
Base Sequence
Bicuculline/pharmacology
Bridged Bicyclo Compounds/pharmacology
Bridged Bicyclo Compounds, Heterocyclic
Cloning, Molecular
Convulsants/pharmacology
DNA Primers
DNA, Complementary/analysis
Drosophila melanogaster/genetics,metabolism
Female
Flunitrazepam/pharmacology
Gene Expression
Macromolecular Substances
Molecular Sequence Data
Muscimol/pharmacology
Oocytes/drug effects,physiology
Pentobarbital/pharmacology
Picrotoxin/pharmacology
Polymerase Chain Reaction
Receptors, GABA/biosynthesis,physiology
Receptors, GABA-A/physiology
Transcription, Genetic
Xenopus laevis
gamma-Aminobutyric Acid/pharmacology
Chemicals
Bridged Bicyclo Compounds
Bridged Bicyclo Compounds, Heterocyclic
Convulsants
DNA Primers
DNA, Complementary
Macromolecular Substances
Receptors, GABA
Receptors, GABA-A
Picrotoxin
Muscimol
gamma-Aminobutyric Acid
Flunitrazepam
tert-butylbicyclophosphorothionate
Pentobarbital
Bicuculline
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chen R
CoCensys, Inc., Irvine, CA 92718.
Belelli D
Lambert J J
Peters J A
Reyes A
Lan N C
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