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PMID: 15757514 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion.

BMC microbiology ·Vol. 5 ·2005-03-09 ·Pages 9

Pallen MJ, Beatson SA, Bailey CM

Abstract

Like many other pathogens, enterohaemorrhagic and enteropathogenic strains of Escherichia coli employ a type-III secretion system to translocate bacterial effector proteins into host cells, where they then disrupt a range of cellular functions. This system is encoded by the locus for enterocyte effacement. Many of the genes within this locus have been assigned names and functions through homology with the better characterised Ysc-Yop system from Yersinia spp. However, the functions and homologies of many LEE genes remain obscure. We have performed a fresh bioinformatics analysis of the LEE. Using PSI-BLAST we have been able to identify several novel homologies between LEE-encoded and Ysc-Yop-associated proteins: Orf2/YscE, Orf5/YscL, rORF8/EscI, SepQ/YscQ, SepL/YopN-TyeA, CesD2/LcrR. In addition, we highlight homology between EspA and flagellin, and report many new homologues of the chaperone CesT. We conclude that the vast majority of LEE-encoded proteins do indeed possess homologues and that homology data can be used in combination with experimental data to make fresh functional predictions.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Computational Biology Enterocytes/metabolism Escherichia coli/genetics,metabolism Gene Deletion Gene Expression Regulation, Bacterial Humans Molecular Sequence Data Protein Structure, Tertiary Sequence Alignment Sequence Homology, Amino Acid Yersinia enterocolitica/genetics,metabolism
Chemicals
Bacterial Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pallen Mark J
Bacterial Pathogenesis and Genomics Unit, Division of Immunity and Infection, Medical School, University of Birmingham, Birmingham, B15 2TT, UK. m.pallen@bham.ac.uk
Beatson Scott A
Bailey Christopher M
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Article Info
Journal
BMC microbiology
Abbr.
BMC Microbiol
ISSN
1471-2180
DOI
10.1186/1471-2180-5-9
Email
m.pallen@bham.ac.uk
Published
2005-03-09
Epub
2005-00-09
Pages
9
Language
English
Region
England
NLM ID
100966981
PMCID
PMC1084347
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2180-5-9 PMC Full Text View journal details
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