Home LiteratureArticle Details

PMID: 3980086 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the temperature-inducible outer membrane proteins of yersiniae.

Infection and immunity ·Vol. 48 ·No. 1 ·1985-04-00 ·Pages 234-40

Bölin I, Portnoy DA, Wolf-Watz H

Abstract

The expression of the temperature-inducible plasmid-coded outer membrane proteins (YOPs) of Yersinia pseudotuberculosis was studied. These proteins were not recovered in the outer membrane fraction when the strain was grown in minimal medium at 37 degrees C, but they were expressed under these conditions. A strict correlation was found between Ca2+ dependency in the virulent strain, YPIII(pIB1), and ability to express YOPs. Ca2+-independent plasmid mutants or RNA-polymerase mutants harboring the virulence plasmid were unable to express YOPs, in contrast to the wild-type strain. These strains were also found to be avirulent. Sera recovered from patients or animals undergoing infection with either Y. pseudotuberculosis, Y. pestis, or Y. enterocolitica possessed antibodies directed against YOPs, indicating that they were expressed in all three pathogenic Yersinia species during infection. The YOPs of the three different species showed high immunological relatedness.

MeSH Terms

Animals Antibodies, Bacterial/analysis Bacterial Outer Membrane Proteins/analysis Calcium/pharmacology Humans Mice Plasmids Temperature Terminology as Topic Virulence Yersinia/analysis,pathogenicity Yersinia Infections/metabolism

Chemicals

Antibodies, Bacterial Bacterial Outer Membrane Proteins Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bölin I

Portnoy D A

Wolf-Watz H

References (26)

26 references, click to expand

Growth of Pasteurella pseudotuberculosis in simulated intracellular and extracellular environments.
J Infect Dis. 1967 Dec;117(5):403-17 PMID: 6078220
Vwa+ phenotype of Yersinia enterocolitica.
Infect Immun. 1983 Apr;40(1):166-71 PMID: 6832830
The genus Yersinia: biochemistry and genetics of virulence.
Curr Top Microbiol Immunol. 1972;57:111-58 PMID: 4554354
Plasmid-mediated and temperature-regulated surface properties of Yersinia enterocolitica.
Infect Immun. 1983 Sep;41(3):921-30 PMID: 6885171
Characterization of common virulence plasmids in Yersinia species and their role in the expression of outer membrane proteins.
Infect Immun. 1984 Jan;43(1):108-14 PMID: 6317562
Molecular cloning of the temperature-inducible outer membrane protein 1 of Yersinia pseudotuberculosis.
Infect Immun. 1984 Jan;43(1):72-8 PMID: 6317574
Virulence plasmid-associated autoagglutination in Yersinia spp.
J Bacteriol. 1984 Jun;158(3):1033-6 PMID: 6725209
Transfer of the virulence plasmid of Yersinia pestis to Yersinia pseudotuberculosis.
Infect Immun. 1985 Apr;48(1):241-3 PMID: 3884511
The basis of virulence in Pasteurella pestis: an antigen determining virulence.
Br J Exp Pathol. 1956 Oct;37(5):481-93 PMID: 13374206
Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence.
J Bacteriol. 1961 Apr;81:605-8 PMID: 13714203
THE EFFECT OF CA++ AND MG++ ON LYSIS, GROWTH, AND PRODUCTION OF VIRULENCE ANTIGENS BY PASTEURELLA PESTIS.
J Infect Dis. 1964 Feb;114:13-25 PMID: 14118042
VIRULENCE OF PASTEURELLA PESTIS AND IMMUNITY TO PLAGUE.
Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1963;37:59-113 PMID: 14324291
V and W antigens in strains of Pasteurella pseudotuberculosis.
Br J Exp Pathol. 1960 Feb;41:38-44 PMID: 13806301
Plasmid-mediated tissue invasiveness in Yersinia enterocolitica.
Nature. 1980 Jan 10;283(5743):224-6 PMID: 7350546
Correlation of autoagglutination and virulence of yersiniae.
J Clin Microbiol. 1980 Apr;11(4):430-2 PMID: 7372804
Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica.
Infect Immun. 1980 Feb;27(2):682-5 PMID: 7380546
Plague virulence antigens from Yersinia enterocolitica.
Infect Immun. 1980 May;28(2):638-40 PMID: 7399681
Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis.
Infect Immun. 1980 Jun;28(3):1044-7 PMID: 6249747
Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.
Infect Immun. 1981 Feb;31(2):775-82 PMID: 7216474
Plasmids in Yersinia pestis.
Infect Immun. 1981 Feb;31(2):839-41 PMID: 7216478
Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment.
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1224-8 PMID: 6940138
Essential virulence determinants of different Yersinia species are carried on a common plasmid.
Plasmid. 1981 Mar;5(2):183-7 PMID: 7243971
Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis.
J Bacteriol. 1981 Dec;148(3):877-83 PMID: 6273385
Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid.
Infect Immun. 1982 Aug;37(2):506-12 PMID: 6749681
Antigenicity of Neisseria gonorrhoeae outer membrane protein(s) III detected by immunoprecipitation and Western blot transfer with a monoclonal antibody.
Infect Immun. 1982 Nov;38(2):668-72 PMID: 6183218
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.48.1.234-240.1985

Published

1985-04-00

Pages

234-40

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC261940

Subset

External Links

PubMed source DOI: 10.1128/iai.48.1.234-240.1985 PMC Full Text View journal details

Analysis Services

Expression of the temperature-inducible outer membrane proteins of yersiniae.

Contact

WeChat Official Account