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PMID: 15175293 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Quorum sensing regulates type III secretion in Vibrio harveyi and Vibrio parahaemolyticus.

Journal of bacteriology ·Vol. 186 ·No. 12 ·2004-06-00 ·Pages 3794-805

Henke JM, Bassler BL

Abstract

In a process known as quorum sensing, bacteria communicate with one another by producing, releasing, detecting, and responding to signal molecules called autoinducers. Vibrio harveyi, a marine pathogen, uses two parallel quorum-sensing circuits, each consisting of an autoinducer-sensor pair, to control the expression of genes required for bioluminescence and a number of other target genes. Genetic screens designed to discover autoinducer-regulated targets in V. harveyi have revealed genes encoding components of a putative type III secretion (TTS) system. Using transcriptional reporter fusions and TTS protein localization studies, we show that the TTS system is indeed functional in V. harveyi and that expression of the genes encoding the secretion machinery requires an intact quorum-sensing signal transduction cascade. The newly completed genome of the closely related marine bacterium Vibrio parahaemolyticus, which is a human pathogen, shows that it possesses the genes encoding both of the V. harveyi-like quorum-sensing signaling circuits and that it also has a TTS system similar to that of V. harveyi. We show that quorum sensing regulates TTS in V. parahaemolyticus. Previous reports connecting quorum sensing to TTS in enterohemorrhagic and enteropathogenic Escherichia coli show that quorum sensing activates TTS at high cell density. Surprisingly, we find that at high cell density (in the presence of autoinducers), quorum sensing represses TTS in V. harveyi and V. parahaemolyticus.

MeSH Terms
4-Butyrolactone/analogs & derivatives,metabolism Bacterial Proteins/genetics,metabolism Culture Media, Conditioned Gene Expression Regulation, Bacterial Luminescent Measurements Molecular Sequence Data Multigene Family Repressor Proteins/genetics,metabolism Sequence Analysis, DNA Signal Transduction Transcription, Genetic Vibrio/genetics,growth & development,metabolism Vibrio parahaemolyticus/genetics,growth & development,metabolism
Chemicals
Bacterial Proteins Culture Media, Conditioned Repressor Proteins N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone 4-Butyrolactone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Henke Jennifer M
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA.
Bassler Bonnie L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.12.3794-3805.2004
Published
2004-06-00
Pages
3794-805
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC419960
Subset
IM
Grants
NIGMS NIH HHS · R01 GM065859 · United States
NIGMS NIH HHS · R01 GM 065859 · United States
Databases
GENBANK
AY524044
Corrections
CommentIn
PMID: 15175279
External Links
PubMed source DOI: 10.1128/JB.186.12.3794-3805.2004 PMC Full Text View journal details
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