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PMID: 15060055 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB.

Journal of bacteriology ·Vol. 186 ·No. 8 ·2004-04-00 ·Pages 2495-8

Van Arnam JS, McMurry JL, Kihara M, Macnab RM

Abstract

Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.

MeSH Terms
Bacterial Proteins/analysis,genetics Blotting, Western Flagella/metabolism Genetic Complementation Test Membrane Proteins/analysis,genetics Salmonella/metabolism
Chemicals
Bacterial Proteins FlhB protein, Bacteria FliR protein, Bacteria Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Van Arnam John S
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
McMurry Jonathan L
Kihara May
Macnab Robert M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.8.2495-2498.2004
Published
2004-04-00
Pages
2495-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC412181
Subset
IM
Grants
NIAID NIH HHS · R01 AI012202 · United States
NIAID NIH HHS · AI12202 · United States
External Links
PubMed source DOI: 10.1128/JB.186.8.2495-2498.2004 PMC Full Text View journal details
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