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PMID: 10809678 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Deletion analysis of the flagellar switch protein FliG of Salmonella.

Journal of bacteriology ·Vol. 182 ·No. 11 ·2000-06-00 ·Pages 3022-8

Kihara M, Miller GU, Macnab RM

Abstract

The flagellar motor/switch complex, consisting of the three proteins FliG, FliM, and FliN, plays a central role in bacterial motility and chemotaxis. We have analyzed FliG, using 10-amino-acid deletions throughout the protein and testing the deletion clones for their motility and dominance properties and for interaction of the deletion proteins with the MS ring protein FliF. Only the N-terminal 46 amino acids of FliG (segments 1 to 4) were important for binding to FliF; consistent with this, an N-terminal fragment consisting of residues 1 to 108 bound FliF strongly, whereas a C-terminal fragment consisting of residues 109 to 331 did not bind FliF at all. Deletions in the region from residues 37 to 96 (segments 4 to 9), 297 to 306 (segment 30), and 317 to 326 (segment 32) permitted swarming, though not at wild-type levels; all other deletions caused paralyzed or, more commonly, nonflagellate phenotype. Except for those near the N terminus, deletions had a dominant negative effect on wild-type cells.

MeSH Terms
Bacterial Proteins/genetics,metabolism Chemotaxis DNA Mutational Analysis Flagella/genetics Genetic Complementation Test Membrane Proteins Molecular Motor Proteins/genetics Movement Mutation Protein Binding Salmonella/genetics Sequence Deletion
Chemicals
Bacterial Proteins FliN protein, Bacteria Flif protein, Bacteria Flig protein, Bacteria Membrane Proteins Molecular Motor Proteins FliM protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kihara M
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA.
Miller G U
Macnab R M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
2000-06-00
Pages
3022-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94485
Subset
IM
Grants
NIGMS NIH HHS · GM40035 · United States
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