Abstract
The flagellar motor/switch complex, consisting of the three proteins FliG, FliM, and FliN, plays a central role in bacterial motility and chemotaxis. We have analyzed FliG, using 10-amino-acid deletions throughout the protein and testing the deletion clones for their motility and dominance properties and for interaction of the deletion proteins with the MS ring protein FliF. Only the N-terminal 46 amino acids of FliG (segments 1 to 4) were important for binding to FliF; consistent with this, an N-terminal fragment consisting of residues 1 to 108 bound FliF strongly, whereas a C-terminal fragment consisting of residues 109 to 331 did not bind FliF at all. Deletions in the region from residues 37 to 96 (segments 4 to 9), 297 to 306 (segment 30), and 317 to 326 (segment 32) permitted swarming, though not at wild-type levels; all other deletions caused paralyzed or, more commonly, nonflagellate phenotype. Except for those near the N terminus, deletions had a dominant negative effect on wild-type cells.
MeSH Terms
Bacterial Proteins/genetics,metabolism
Chemotaxis
DNA Mutational Analysis
Flagella/genetics
Genetic Complementation Test
Membrane Proteins
Molecular Motor Proteins/genetics
Movement
Mutation
Protein Binding
Salmonella/genetics
Sequence Deletion
Chemicals
Bacterial Proteins
FliN protein, Bacteria
Flif protein, Bacteria
Flig protein, Bacteria
Membrane Proteins
Molecular Motor Proteins
FliM protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kihara M
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA.
Miller G U
Macnab R M
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