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PMID: 3313394 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of the M-ring protein of the flagellar motor of Salmonella typhimurium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 21 ·1987-11-00 ·Pages 7483-7

Homma M, Aizawa S, Dean GE, Macnab RM

Abstract

The M ring is a substructure of the flagellar basal body of bacteria, which lies in the cytoplasmic membrane and is therefore close to the site where the energy of the transmembrane proton potential is converted into mechanical work of rotation of the motor. The protein from which this ring is constructed has not been identified. Flagellar hook-basal body complexes from Salmonella typhimurium were used as the immunogen for the preparation of monoclonal antibodies. An antibody obtained was directed against a major basal-body component, a 65-kDa protein that from mutant studies has been assigned as the product of the flaAII.1 gene. By immunoelectron microscopy, the antibody was observed to bind the innermost feature of the basal body: the cytoplasmic-facing surface of the M ring. We conclude that the 65-kDa protein is a component--probably the main component--of this important substructure of the flagellar motor.

MeSH Terms
Antibodies Antibodies, Monoclonal Bacterial Proteins/analysis,immunology Flagella/analysis,ultrastructure Immunoassay Microscopy, Electron Salmonella typhimurium/analysis,ultrastructure
Chemicals
Antibodies Antibodies, Monoclonal Bacterial Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Homma M
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Aizawa S
Dean G E
Macnab R M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.21.7483
Published
1987-11-00
Pages
7483-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299320
Subset
IM
Grants
NIAID NIH HHS · AI12202 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.21.7483 PMC Full Text View journal details
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