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PMID: 3546266 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization and stoichiometry of hook-associated proteins within Salmonella typhimurium flagella.

Journal of bacteriology ·Vol. 169 ·No. 3 ·1987-03-00 ·Pages 1168-73

Ikeda T, Homma M, Iino T, Asakura S, Kamiya R

Abstract

The localization of hook-associated proteins (HAP1, HAP2, and HAP3) in Salmonella typhimurium flagella was studied by using specific antibodies together with a second antibody conjugated with colloidal gold. HAP1 and HAP3 were localized at the hook-filament junction, as has been suggested previously. HAP2, however, was localized at the filament tip. This finding supports the idea that HAP2 acts to induce polymerization of endogenous flagellin at the filament tip, and HAP1 and HAP3 are junction proteins to connect hook with filament. Analysis of the protein composition of short flagella from a mutant indicated that a single flagellum contains about 10 to 20 HAP1, 10 to 20 HAP2, and 10 to 40 HAP3 molecules.

MeSH Terms
Antibodies Bacterial Proteins/analysis,metabolism Flagella/ultrastructure Immunoassay Microscopy, Electron Mutation Salmonella typhimurium/ultrastructure
Chemicals
Antibodies Bacterial Proteins hook protein, bacterial flagellum
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ikeda T
Homma M
Iino T
Asakura S
Kamiya R
References (25)
25 references, click to expand
  1. Structural genes for flagellar hook-associated proteins in Salmonella typhimurium.
    J Bacteriol. 1985 Aug;163(2):464-71 PMID: 2991190
  2. "Cap" on the tip of Salmonella flagella.
    J Mol Biol. 1985 Aug 20;184(4):735-7 PMID: 4046031
  3. Excretion of unassembled hook-associated proteins by Salmonella typhimurium.
    J Bacteriol. 1985 Dec;164(3):1370-2 PMID: 3905770
  4. In vitro reconstitution of flagellar filaments onto hooks of filamentless mutants of Salmonella typhimurium by addition of hook-associated proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):6169-73 PMID: 3526353
  5. RECONSTITUTION OF BACTERIAL FLAGELLA IN VITRO.
    J Mol Biol. 1964 Oct;10:42-56 PMID: 14222895
  6. Polarity of flagellar growth in salmonella.
    J Gen Microbiol. 1969 May;56(2):227-39 PMID: 4893344
  7. Bacterial flagella: polarity of elongation.
    Science. 1970 Jul 10;169(3941):190-2 PMID: 4987789
  8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  9. Unidirectional growth of Salmonella flagella in vitro.
    J Mol Biol. 1968 Jul 14;35(1):227-36 PMID: 5760562
  10. Structure of straight flagella from a mutant Salmonella.
    J Mol Biol. 1972 Sep 14;70(1):133-52 PMID: 4561344
  11. Copurification of actin and desmin from chicken smooth muscle and their copolymerization in vitro to intermediate filaments.
    J Cell Biol. 1979 Jan;80(1):166-82 PMID: 570568
  12. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  13. Three-dimensional image reconstruction of straight flagella from a mutant Salmonella typhimurium.
    J Mol Biol. 1979 Jul 5;131(3):485-507 PMID: 390160
  14. A new method for determination of parity in optical diffraction patterns from the structures with helical symmetry.
    J Mol Biol. 1980 Mar 15;137(4):437-42 PMID: 7021850
  15. Reconstitution in vitro of flagellar filaments onto hook structures attached to bacterial cells.
    J Mol Biol. 1981 Dec 5;153(2):465-70 PMID: 7040685
  16. Excretion of flagellin by a short-flagella mutant of Salmonella typhimurium.
    J Bacteriol. 1983 Jan;153(1):506-10 PMID: 6336739
  17. Reconstruction in vitro of the flagellar polyhook from Salmonella.
    J Mol Biol. 1982 Nov 15;161(4):551-60 PMID: 7154090
  18. Flagellar hook structures of Caulobacter and Salmonella and their relationship to filament structure.
    J Mol Biol. 1982 Nov 25;162(1):69-87 PMID: 6759665
  19. Motility development of Salmonella typhimurium cells with flaV mutations after addition of exogenous flagellin.
    J Bacteriol. 1983 Jul;155(1):435-7 PMID: 6345512
  20. Hook-associated proteins essential for flagellar filament formation in Salmonella typhimurium.
    J Bacteriol. 1984 Jan;157(1):100-8 PMID: 6360991
  21. Bacterial motility and the bacterial flagellar motor.
    Annu Rev Biophys Bioeng. 1984;13:51-83 PMID: 6378075
  22. In vitro polymerization of flagellin excreted by a short-flagellum Salmonella typhimurium mutant.
    J Bacteriol. 1984 Aug;159(2):787-9 PMID: 6378893
  23. Excretion of unassembled flagellin by Salmonella typhimurium mutants deficient in hook-associated proteins.
    J Bacteriol. 1984 Sep;159(3):1056-9 PMID: 6384179
  24. Ca2+-dependent contraction of human lung fibroblasts treated with Triton X-100: a role of Ca2+-calmodulin-dependent phosphorylation of myosin 20,000-dalton light chain.
    Cell Motil. 1984;4(5):315-31 PMID: 6334557
  25. Locations of hook-associated proteins in flagellar structures of Salmonella typhimurium.
    J Bacteriol. 1985 Apr;162(1):183-9 PMID: 3884587
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.3.1168-1173.1987
Published
1987-03-00
Pages
1168-73
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211915
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.3.1168-1173.1987 PMC Full Text View journal details
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