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PMID: 6090403 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli.

Journal of bacteriology ·Vol. 159 ·No. 3 ·1984-09-00 ·Pages 991-9

Dean GE, Macnab RM, Stader J, Matsumura P, Burks C

Abstract

The motA and motB gene products of Escherichia coli are integral membrane proteins necessary for flagellar rotation. We determined the DNA sequence of the region containing the motA gene and its promoter. Within this sequence, there is an open reading frame of 885 nucleotides, which with high probability (98% confidence level) meets criteria for a coding sequence. The 295-residue amino acid translation product had a molecular weight of 31,974, in good agreement with the value determined experimentally by gel electrophoresis. The amino acid sequence, which was quite hydrophobic, was subjected to a theoretical analysis designed to predict membrane-spanning alpha-helical segments of integral membrane proteins; four such hydrophobic helices were predicted by this treatment. Additional amphipathic helices may also be present. A remarkable feature of the sequence is the existence of two segments of high uncompensated charge density, one positive and the other negative. Possible organization of the protein in the membrane is discussed. Asymmetry in the amino acid composition of translated DNA sequences was used to distinguish between two possible initiation codons. The use of this method as a criterion for authentication of coding regions is described briefly in an Appendix.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics,physiology Bacterial Proteins/genetics Base Sequence Codon DNA Restriction Enzymes Escherichia coli/genetics,physiology Flagella/physiology Genes Genes, Bacterial Genotype Lipid Bilayers Protein Conformation Rotation
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Codon Lipid Bilayers DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dean G E
Macnab R M
Stader J
Matsumura P
Burks C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.3.991-999.1984
Published
1984-09-00
Pages
991-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215758
Subset
IM
Grants
NIAID NIH HHS · AI12202 · United States
NIAID NIH HHS · AI18985 · United States
Databases
GENBANK
J01652
External Links
PubMed source DOI: 10.1128/jb.159.3.991-999.1984 PMC Full Text View journal details
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