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PMID: 1569949 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of a 91-kilodalton PEA3-binding protein is down-regulated during differentiation of F9 embryonal carcinoma cells.

Molecular and cellular biology ·Vol. 12 ·No. 5 ·1992-05-00 ·Pages 2213-21

Martin ME, Yang XY, Folk WR

Abstract

Proteins binding to the PEA3 enhancer motif (AGGAAG) activate the polyomavirus early promoter and help comprise the viral late mRNA initiator element (W. Yoo, M. E. Martin, and W. R. Folk, J. Virol. 65:5391-5400, 1991). Because many developmentally regulated cellular genes have PEA3 motifs near their promoter sequences, and because Ets family gene products activate the PEA3 motif, we have studied the expression of PEA3-binding proteins and Ets-related proteins during differentiation of F9 embryonal carcinoma cells. An approximately 91-kDa protein (PEA3-91) was identified in F9 cell nuclear extracts by UV cross-linking to a radiolabeled PEA3 oligonucleotide probe, and expression of PEA3-91 was down-regulated after differentiation of F9 cells to parietal endoderm. The c-ets-1 gene product binds to a sequence in the murine sarcoma virus long terminal repeat that is similar to the PEA3 motif (cGGAAG), but PEA3-91 was not cross-linked to this Ets-1-binding motif, nor did antiserum which recognizes murine c-ets-1 and c-ets-2 proteins have any effect on PEA3-binding activity in mobility shift assays. Furthermore, c-ets-1 mRNA was not detected in undifferentiated or differentiated F9 cells, and c-ets-2 mRNA levels remained high after differentiation. Antiserum against the Drosophila Ets-related E74A protein, however, recognized an approximately 92-kDa protein in F9 cells whose expression during differentiation varied in a manner identical to that of PEA3-91. These data suggest that PEA3-91 is not the product of the ets-1 or ets-2 genes but is likely to be the product of a murine homolog of the Drosophila E74 gene.

Related Genes
c-ets-1 c-ets-2
MeSH Terms
Animals Base Sequence Cell Differentiation Cell Line Cell Nucleus/metabolism DNA-Binding Proteins/genetics,metabolism,radiation effects Drosophila Enhancer Elements, Genetic Gene Expression Regulation, Neoplastic Mice Molecular Sequence Data Oligodeoxyribonucleotides/metabolism Protein-Tyrosine Kinases/genetics Proto-Oncogene Protein c-ets-1 Proto-Oncogene Protein c-ets-2 Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-ets RNA, Messenger/genetics,isolation & purification RNA, Neoplasm/genetics,isolation & purification Repressor Proteins Teratoma Trans-Activators Transcription Factors/genetics,metabolism Ultraviolet Rays
Chemicals
DNA-Binding Proteins ERF protein, human ETS1 protein, human Ets1 protein, mouse Ets2 protein, mouse Oligodeoxyribonucleotides Proto-Oncogene Protein c-ets-1 Proto-Oncogene Protein c-ets-2 Proto-Oncogene Proteins Proto-Oncogene Proteins c-ets RNA, Messenger RNA, Neoplasm Repressor Proteins Trans-Activators Transcription Factors enhancer-binding protein AP-3 Protein-Tyrosine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martin M E
Department of Biochemistry, University of Missouri, Columbia 65212.
Yang X Y
Folk W R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.5.2213-2221.1992
Published
1992-05-00
Pages
2213-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364393
Subset
IM
Grants
NCI NIH HHS · CA-45033 · United States
NICHD NIH HHS · HD-27200 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.5.2213-2221.1992 PMC Full Text View journal details
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