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PMID: 15692568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Central role of Ifh1p-Fhl1p interaction in the synthesis of yeast ribosomal proteins.

The EMBO journal ·Vol. 24 ·No. 3 ·2005-02-09 ·Pages 533-42

Rudra D, Zhao Y, Warner JR

Abstract

The 138 genes encoding the 79 ribosomal proteins (RPs) of Saccharomyces cerevisiae form the tightest cluster of coordinately regulated genes in nearly all transcriptome experiments. The basis for this observation remains unknown. We now provide evidence that two factors, Fhl1p and Ifh1p, are key players in the transcription of RP genes. Both are found at transcribing RP genes in vivo. Ifh1p, but not Fhl1p, leaves the RP genes when transcription is repressed. The occupancy of the RP genes by Ifh1p depends on its interaction with the phospho-peptide recognizing forkhead-associated domain of Fhl1p. Disruption of this interaction is severely deleterious to ribosome synthesis and cell growth. Loss of functional Fhl1p leads to cells that have only 20% the normal amount of RNA and that synthesize ribosomes at only 5-10% the normal rate. Homeostatic mechanisms within the cell respond by reducing the transcription of rRNA to match the output of RPs, and by reducing the global transcription of mRNA to match the capacity of the translational apparatus.

MeSH Terms
Cell Division DNA, Fungal/genetics,metabolism Forkhead Transcription Factors Genes, Fungal Mutagenesis, Site-Directed Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic Protein Binding Protein Structure, Tertiary RNA, Fungal/genetics,metabolism RNA, Messenger/genetics,metabolism Recombinant Proteins/chemistry,genetics,metabolism Ribosomal Proteins/biosynthesis,genetics Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/biosynthesis,chemistry,genetics,metabolism Trans-Activators/chemistry,genetics,metabolism Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic
Chemicals
DNA, Fungal FHL1 protein, S cerevisiae Forkhead Transcription Factors IFH1 protein, S cerevisiae RNA, Fungal RNA, Messenger Recombinant Proteins Ribosomal Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rudra Dipayan
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Zhao Yu
Warner Jonathan R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600553
Published
2005-02-09
Epub
2005-00-03
Pages
533-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC548658
Subset
IM
Grants
NIGMS NIH HHS · R01 GM025532 · United States
NCI NIH HHS · CA-13330 · United States
NIGMS NIH HHS · GM-25532 · United States
External Links
PubMed source DOI: 10.1038/sj.emboj.7600553 PMC Full Text View journal details
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