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PMID: 12655405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

The different (sur)faces of Rap1p.

Molecular genetics and genomics : MGG ·Vol. 268 ·No. 6 ·2003-03-00 ·Pages 791-8

Piña B, Fernández-Larrea J, García-Reyero N, Idrissi FZ

Abstract

The DNA-binding protein Rap1p fulfills many different functions in the yeast cell. It targets 5% of the promoters, acting both as a transcriptional activator and as a repressor, depending on the DNA sequence context. In addition, Rap1p is an essential structural component of yeast telomeres, where it contributes to telomeric silencing. Here we review the evidence indicating that Rap1p function is modulated by the precise architecture of the its binding site and its surroundings: long tracts of telomeric repeats for telomeric functions, specific sequences and orientation for maximal transcriptional activation, and specific DNA recognition sequences for complementary factors in other cases. Many of these functions are probably related to chromatin organization around Rap1p DNA binding sites, resulting from the very tight binding of Rap1p to DNA. We propose that Rap1p alters its structure to bind to different versions of its DNA binding sequence. These structural changes may modulate the function of Rap1p domains, providing different interacting surfaces for binding to specific co-operating factors, and thus contributing to the diversity of Rap1p function.

MeSH Terms
Base Sequence Binding Sites/genetics DNA, Fungal/genetics,metabolism Genes, Fungal Models, Biological Promoter Regions, Genetic Protein Structure, Tertiary Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/chemistry,genetics,metabolism Shelterin Complex Telomere/genetics,metabolism Telomere-Binding Proteins/chemistry,genetics,metabolism Transcription Factors/chemistry,genetics,metabolism
Chemicals
DNA, Fungal RAP1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Shelterin Complex Telomere-Binding Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Piña B
Molecular and Cellular Biology Department, Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Jordi Girona 18, Spain. bpcbmc@cid.csic.es
Fernández-Larrea J
García-Reyero N
Idrissi F-Z
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-002-0801-3
Email
bpcbmc@cid.csic.es
Published
2003-03-00
Epub
2003-00-25
Pages
791-8
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-002-0801-3 View journal details
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