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PMID: 15545630 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

MIM/BEG4, a Sonic hedgehog-responsive gene that potentiates Gli-dependent transcription.

Genes & development ·Vol. 18 ·No. 22 ·2004-11-15 ·Pages 2724-9

Callahan CA, Ofstad T, Horng L, Wang JK, Zhen HH, Coulombe PA, Oro AE

Abstract

Sonic hedgehog (Shh) signaling plays a critical role during development and carcinogenesis. While Gli family members govern the transcriptional output of Shh signaling, little is known how Gli-mediated transcriptional activity is regulated. Here we identify the actin-binding protein Missing in Metastasis (MIM) as a new Shh-responsive gene. Together, Gli1 and MIM recapitulate Shh-mediated epidermal proliferation and invasion in regenerated human skin. MIM is part of a Gli/Suppressor of Fused complex and potentiates Gli-dependent transcription using domains distinct from those used for monomeric actin binding. These data define MIM as both a Shh-responsive gene and a new member of the pathway that modulates Gli responses during growth and tumorigenesis.

MeSH Terms
Actins/metabolism Animals Cell Division Embryo, Mammalian/cytology,metabolism Epidermal Cells Epidermis/metabolism Fibroblasts/cytology,metabolism Gene Expression Profiling Hedgehog Proteins Humans Immunoprecipitation In Situ Hybridization Kruppel-Like Transcription Factors Mice Mice, Knockout Microfilament Proteins/genetics,immunology,pharmacology Neoplasm Invasiveness/pathology Neoplasm Proteins/genetics,immunology,pharmacology Nuclear Proteins Oligonucleotide Array Sequence Analysis Protein Binding Protein Structure, Tertiary Rabbits Recombinant Fusion Proteins/metabolism Regeneration Repressor Proteins/metabolism Skin/cytology,metabolism Trans-Activators/metabolism Transcription Factors/antagonists & inhibitors,genetics,metabolism Transcription, Genetic Zinc Finger Protein GLI1 Zinc Finger Protein Gli2 Zinc Fingers
Chemicals
Actins GLI1 protein, human GLI2 protein, human Gli1 protein, mouse Gli2 protein, mouse Hedgehog Proteins Kruppel-Like Transcription Factors Microfilament Proteins Mtss1 protein, mouse Neoplasm Proteins Nuclear Proteins Recombinant Fusion Proteins Repressor Proteins SHH protein, human Sufu protein, mouse Trans-Activators Transcription Factors Zinc Finger Protein GLI1 Zinc Finger Protein Gli2
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Callahan Christopher A
Program in Epithelial Biology and Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Ofstad Tyler
Horng Lily
Wang Jordon K
Zhen Hanson H
Coulombe Pierre A
Oro Anthony E
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1221804
Published
2004-11-15
Pages
2724-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC528890
Subset
IM
Grants
NIAMS NIH HHS · R01 AR44232 · United States
NIAMS NIH HHS · P01 AR044012 · United States
NIAMS NIH HHS · K08 AR047803 · United States
NIAMS NIH HHS · R01 AR46786-02 · United States
NIAMS NIH HHS · R01 AR044232 · United States
NIAMS NIH HHS · R01 AR046786 · United States
NIAMS NIH HHS · P01AR44012 · United States
External Links
PubMed source DOI: 10.1101/gad.1221804 PMC Full Text View journal details
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