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PMID: 11445535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor.

Genes & development ·Vol. 15 ·No. 13 ·2001-07-01 ·Pages 1613-8

Prokhortchouk A, Hendrich B, Jørgensen H, Ruzov A, Wilm M, Georgiev G, Bird A, Prokhortchouk E

Abstract

We describe a novel mammalian DNA binding activity that requires at least two symmetrically methylated CpG dinucleotides in its recognition sequence, preferably within the sequence 5'CGCG. A key component of the activity is Kaiso, a protein with POZ and zinc-finger domains that is known to associate with p120 catenin. We find that Kaiso behaves as a methylation-dependent transcriptional repressor in transient transfection assays. Kaiso is a constituent of one of two methyl-CpG binding complexes originally designated as MeCP1. The data suggest that zinc-finger motifs are responsible for DNA binding, and may therefore target repression to specific methylated regions of the genome. As Kaiso associates with p120 catenin, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing.

MeSH Terms
Animals Catenins Cell Adhesion Molecules/metabolism Cell Extracts Cell Line Cell Nucleus/metabolism CpG Islands DNA Methylation DNA-Binding Proteins/genetics,metabolism Histone Deacetylases Humans Mice Phosphoproteins/metabolism Rabbits Repressor Proteins/genetics,metabolism Signal Transduction Transcription Factors/genetics,metabolism Transcription, Genetic Zinc Fingers
Chemicals
Catenins Cell Adhesion Molecules Cell Extracts DNA-Binding Proteins MeCP1 histone deacetylase complex, human MeCP1 histone deacetylase complex, mouse Phosphoproteins Repressor Proteins Transcription Factors ZBTB33 protein, human Zbtb33 protein, mouse delta catenin Histone Deacetylases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Prokhortchouk A
Group of Transcriptional Control and Oncogenesis, Institute of Gene Biology, Moscow, Russia.
Hendrich B
Jørgensen H
Ruzov A
Wilm M
Georgiev G
Bird A
Prokhortchouk E
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.198501
Published
2001-07-01
Pages
1613-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312733
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.198501 PMC Full Text View journal details
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