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PMID: 1537347 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recognition of the adenovirus type 2 origin of DNA replication by the virally encoded DNA polymerase and preterminal proteins.

The EMBO journal ·Vol. 11 ·No. 2 ·1992-02-00 ·Pages 761-8

Temperley SM, Hay RT

Abstract

Initiation of adenovirus DNA synthesis is preceded by the assembly of a nucleoprotein complex at the origin of DNA replication containing three viral proteins, preterminal protein, DNA polymerase and DNA binding protein, and two cellular proteins, nuclear factors I and III. While sequence specific interactions of the cellular proteins with their cognate sites in the origin of DNA replication are well characterized, the question of how the viral replication proteins recognize the origin has remained unanswered. Preterminal protein and DNA polymerase were therefore purified to homogeneity from recombinant baculovirus infected insect cells. Gel filtration demonstrated that while DNA polymerase existed in monomeric and dimeric forms, preterminal protein was predominantly monomeric and when combined the proteins formed a stable heterodimer. In a gel electrophoresis DNA binding assay each of the protein species recognized DNA within the origin of DNA replication with unique specificity. Competition analysis and DNase I protection experiments revealed that although each protein could recognize the origin, the heterodimer did so with enhanced specificity, protecting bases 8-17 from cleavage with the nuclease. Thus the highly conserved 'core' of the origin of DNA replication, present in all human adenoviruses, is recognized by the preterminal protein--DNA polymerase heterodimer.

MeSH Terms
Adenoviruses, Human/enzymology,genetics Animals Baculoviridae Base Sequence Cell Line Chromatography, Gel DNA Replication DNA-Binding Proteins/metabolism DNA-Directed DNA Polymerase/genetics,isolation & purification,metabolism Deoxyribonuclease I Enzyme Precursors/genetics,isolation & purification,metabolism Gene Products, pol/genetics,isolation & purification,metabolism Insecta Kinetics Macromolecular Substances Molecular Sequence Data Oligodeoxyribonucleotides/chemical synthesis Substrate Specificity Transfection
Chemicals
DNA-Binding Proteins Enzyme Precursors Gene Products, pol Macromolecular Substances Oligodeoxyribonucleotides DNA-Directed DNA Polymerase Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Temperley S M
Department of Biochemistry and Microbiology, University of St Andrews, Fife, UK.
Hay R T
References (52)
52 references, click to expand
  1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  2. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  3. Identification of two distinct regions within the adenovirus minimal origin of replication that are required for adenovirus type 4 DNA replication in vitro.
    J Virol. 1991 Sep;65(9):5037-44 PMID: 1870210
  4. Mechanism of action of an acidic transcriptional activator in vitro.
    Cell. 1991 Mar 8;64(5):971-81 PMID: 2001592
  5. Interactions between the adenovirus type 2 DNA polymerase and the DNA binding domain of nuclear factor I.
    New Biol. 1990 Dec;2(12):1083-90 PMID: 2088500
  6. Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin.
    Nature. 1990 Aug 9;346(6284):534-9 PMID: 2165567
  7. Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.
    Nucleic Acids Res. 1990 Mar 11;18(5):1167-73 PMID: 2181402
  8. Protein-protein interactions between adenovirus DNA polymerase and nuclear factor I mediate formation of the DNA replication preinitiation complex.
    J Biol Chem. 1990 Oct 25;265(30):18634-42 PMID: 2211726
  9. Transcription factors NFI and NFIII/oct-1 function independently, employing different mechanisms to enhance adenovirus DNA replication.
    J Virol. 1990 Nov;64(11):5510-8 PMID: 2214023
  10. Expression of enzymatically active adenovirus DNA polymerase from cloned DNA requires sequences upstream of the main open reading frame.
    Virology. 1987 Dec;161(2):520-6 PMID: 3686828
  11. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication.
    Nature. 1986 Aug 14-20;322(6080):656-9 PMID: 3748145
  12. Adenovirus origin of DNA replication: sequence requirements for replication in vitro.
    Mol Cell Biol. 1987 Feb;7(2):864-74 PMID: 3821730
  13. Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication.
    Mol Cell Biol. 1987 Feb;7(2):875-86 PMID: 3821731
  14. The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo.
    EMBO J. 1985 Feb;4(2):421-6 PMID: 4018031
  15. Adenovirus DNA replication in vitro: site-directed mutagenesis of the nuclear factor I binding site of the Ad2 origin.
    Nucleic Acids Res. 1985 Jul 11;13(13):4935-52 PMID: 4040630
  16. Origin of adenovirus DNA replication. Role of the nuclear factor I binding site in vivo.
    J Mol Biol. 1985 Nov 5;186(1):129-36 PMID: 4078897
  17. Replication of origin containing adenovirus DNA fragments that do not carry the terminal protein.
    Nucleic Acids Res. 1983 Apr 11;11(7):1975-89 PMID: 6300787
  18. Template requirements for the initiation of adenovirus DNA replication.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):100-4 PMID: 6320160
  19. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177-81 PMID: 6336326
  20. Introduction, stable integration, and controlled expression of a chimeric adenovirus gene whose product is toxic to the recipient human cell.
    Mol Cell Biol. 1984 Jul;4(7):1354-62 PMID: 6542172
  21. The adenovirus DNA binding protein and adenovirus DNA polymerase interact to catalyze elongation of primed DNA templates.
    J Biol Chem. 1986 Aug 5;261(22):10218-27 PMID: 2942538
  22. Viable viruses with deletions in the left inverted terminal repeat define the adenovirus origin of DNA replication.
    J Gen Virol. 1986 Feb;67 ( Pt 2):321-32 PMID: 3003240
  23. Reconstruction of adenovirus replication origins with a human nuclear factor I binding site.
    J Biol Chem. 1986 Mar 5;261(7):3339-46 PMID: 3005289
  24. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  25. Contactpoint analysis of the HeLa nuclear factor I recognition site reveals symmetrical binding at one side of the DNA helix.
    EMBO J. 1987 Jan;6(1):161-8 PMID: 3034574
  26. Purification of a NF1-like DNA-binding protein from rat liver and cloning of the corresponding cDNA.
    EMBO J. 1988 Oct;7(10):3115-23 PMID: 3053160
  27. DNA replication.
    J Biol Chem. 1988 Jan 5;263(1):1-4 PMID: 3275635
  28. Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro.
    J Virol. 1988 Feb;62(2):496-500 PMID: 3336069
  29. High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus.
    Nucleic Acids Res. 1988 Mar 25;16(6):2431-44 PMID: 3362670
  30. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  31. DNA sequences required for the initiation of adenovirus type 4 DNA replication in vitro.
    J Mol Biol. 1988 May 5;201(1):57-67 PMID: 3418700
  32. A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.
    Nucleic Acids Res. 1986 Feb 11;14(3):1303-17 PMID: 3513122
  33. Initiation of adenovirus DNA replication in vitro requires a specific DNA sequence.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6446-50 PMID: 6579530
  34. DNA sequences required for the in vitro replication of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3069-73 PMID: 6587341
  35. A size analysis of the adenovirus replicon.
    EMBO J. 1984 Feb;3(2):333-7 PMID: 6714222
  36. Structure and function of the adenovirus origin of replication.
    Cell. 1984 May;37(1):309-19 PMID: 6722875
  37. Replication of adenovirus mini-chromosomes.
    J Mol Biol. 1984 Jun 5;175(4):493-510 PMID: 6737476
  38. Function of adenovirus terminal protein in the initiation of DNA replication.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2221-5 PMID: 6954537
  39. Adenovirus DNA-binding protein forms a multimeric protein complex with double-stranded DNA and enhances binding of nuclear factor I.
    J Virol. 1990 Jan;64(1):379-86 PMID: 2293667
  40. Amino-terminal domain of NF1 binds to DNA as a dimer and activates adenovirus DNA replication.
    EMBO J. 1990 Feb;9(2):559-66 PMID: 2303041
  41. The DNA binding domain (POU domain) of transcription factor oct-1 suffices for stimulation of DNA replication.
    EMBO J. 1990 Jun;9(6):1883-8 PMID: 2347308
  42. Interaction of enhancer-binding protein EBP1 (NF-kappa B) with the human immunodeficiency virus type 1 enhancer.
    J Virol. 1990 Mar;64(3):1335-44 PMID: 2406459
  43. The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
    Cell. 1989 Aug 25;58(4):741-53 PMID: 2504497
  44. Structural and functional organization of a porcine gene coding for nuclear factor I.
    Biochemistry. 1989 Oct 3;28(20):8191-200 PMID: 2513876
  45. Animal virus DNA replication.
    Annu Rev Biochem. 1989;58:671-717 PMID: 2549858
  46. Initiation of eukaryotic DNA replication in vitro.
    Annu Rev Cell Biol. 1989;5:197-245 PMID: 2557059
  47. Recognition mechanisms in the synthesis of animal virus DNA.
    Biochem J. 1989 Feb 15;258(1):3-16 PMID: 2649089
  48. Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication.
    EMBO J. 1989 Jun;8(6):1841-8 PMID: 2767055
  49. Purification and characterization of nuclear factor III (origin recognition protein C), a sequence-specific DNA binding protein required for efficient initiation of adenovirus DNA replication.
    J Biol Chem. 1988 Jan 15;263(2):931-7 PMID: 2826468
  50. Initiation of adenovirus DNA replication. II. Structural requirements using synthetic oligonucleotide adenovirus templates.
    J Biol Chem. 1988 Jul 15;263(20):9809-17 PMID: 2838480
  51. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  52. Replication of adenovirus type 4 DNA by a purified fraction from infected cells.
    Nucleic Acids Res. 1991 Jun 25;19(12):3243-9 PMID: 1829516
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-02-00
Pages
761-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556509
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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