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PMID: 2767055 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication.

The EMBO journal ·Vol. 8 ·No. 6 ·1989-06-00 ·Pages 1841-8

Cleat PH, Hay RT

Abstract

The DNA-protein and protein-protein interactions proposed for the stability of nucleoprotein complexes at the origin of replication in prokaryotes are also thought to impart regulatory precision in eukaryotic DNA replication. This type of specificity can be observed, for example, during adenovirus DNA replication where efficient initiation requires that nuclear factor I (NFI) binds to the origin of DNA replication. Addition of purified NFI stimulates the initiation of adenovirus DNA replication in vitro in a reaction that is dependent on the concentration of the adenovirus DNA binding protein (DBP). However, the molecular basis for the synergistic action of NFI and DBP during replication is at present unknown. We report here that DBP increases the affinity of NFI for its binding site in the replication origin. DBP did not, however, increase the affinity of another eukaryotic sequence-specific DNA binding protein, EBP1, for its recognition site. Other single-stranded DNA binding proteins could not substitute for DBP in increasing NFI affinity for its binding site. In addition, DBP was found to alter the binding kinetics of NFI, both by increasing the rate of association and decreasing the rate of dissociation of NFI with the DNA template. The co-operativity between NFI and DBP was also demonstrated on another DNA template, a human NFI site (FIB2), suggesting that this interaction is of general occurrence and not restricted to the adenovirus origin of replication.

MeSH Terms
Adenoviridae/metabolism Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins DNA Replication DNA, Viral/metabolism DNA-Binding Proteins/metabolism Kinetics Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Transcription Factors Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cleat P H
Department of Biochemistry and Microbiology, University of St Andrews, Fife, UK.
Hay R T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-06-00
Pages
1841-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401031
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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