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PMID: 15155892 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein alpha subunit GPA1 and regulates abscisic acid signaling.

The Plant cell ·Vol. 16 ·No. 6 ·2004-06-00 ·Pages 1616-32

Pandey S, Assmann SM

Abstract

Heterotrimeric G proteins composed of alpha, beta, and gamma subunits link ligand perception by G protein-coupled receptors (GPCRs) with downstream effectors, providing a ubiquitous signaling mechanism in eukaryotes. The Arabidopsis thaliana genome encodes single prototypical Galpha (GPA1) and Gbeta (AGB1) subunits, and two probable Ggamma subunits (AGG1 and AGG2). One Arabidopsis gene, GCR1, encodes a protein with significant sequence similarity to nonplant GPCRs and a predicted 7-transmembrane domain structure characteristic of GPCRs. However, whether GCR1 actually interacts with GPA1 was unknown. We demonstrate by in vitro pull-down assays, by yeast split-ubiquitin assays, and by coimmunoprecipitation from plant tissue that GCR1 and GPA1 are indeed physically coupled. GCR1-GPA1 interaction depends on intracellular domains of GCR1. gcr1 T-DNA insertional mutants exhibit hypersensitivity to abscisic acid (ABA) in assays of root growth, gene regulation, and stomatal response. gcr1 guard cells are also hypersensitive to the lipid metabolite, sphingosine-1-phosphate (S1P), which is a transducer of the ABA signal upstream of GPA1. Because gpa1 mutants exhibit insensitivity in aspects of guard cell ABA and S1P responses, whereas gcr1 mutants exhibit hypersensitivity, GCR1 may act as a negative regulator of GPA1-mediated ABA responses in guard cells.

MeSH Terms
Abscisic Acid/pharmacology Arabidopsis/cytology,drug effects,genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism DNA, Bacterial/genetics Desiccation Disasters GTP-Binding Protein alpha Subunits/metabolism Gene Expression Regulation, Plant/drug effects Lysophospholipids/pharmacology Mutagenesis, Insertional/genetics Plant Diseases/genetics Plant Leaves/cytology,drug effects,metabolism Protein Binding Protein Structure, Tertiary Receptors, G-Protein-Coupled/chemistry,genetics,metabolism Sequence Deletion/genetics Signal Transduction/drug effects Sphingosine/analogs & derivatives,pharmacology Ubiquitin/genetics,metabolism Water/analysis,metabolism
Chemicals
Arabidopsis Proteins DNA, Bacterial GCR1 protein, Arabidopsis GPA1 protein, Arabidopsis GTP-Binding Protein alpha Subunits Lysophospholipids Receptors, G-Protein-Coupled T-DNA Ubiquitin Water sphingosine 1-phosphate Abscisic Acid Sphingosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pandey Sona
Biology Department, Pen State University, University Park, Pensylvania, 16802, USA.
Assmann Sarah M
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.020321
Published
2004-06-00
Epub
2004-00-21
Pages
1616-32
Language
English
Region
England
NLM ID
9208688
PMCID
PMC490050
Subset
IM
Corrections
ErratumIn
PMID: 31358647
External Links
PubMed source DOI: 10.1105/tpc.020321 PMC Full Text View journal details
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