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PMID: 11930019 Published · ppublish English Journal Article

GCR1, the putative Arabidopsis G protein-coupled receptor gene is cell cycle-regulated, and its overexpression abolishes seed dormancy and shortens time to flowering.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 7 ·2002-04-02 ·Pages 4736-41

Colucci G, Apone F, Alyeshmerni N, Chalmers D, Chrispeels MJ

Abstract

Although signaling through heterotrimeric G proteins has been extensively studied in eukaryotes, there is little information about this important signaling pathway in plants. We observed that expression of GCR1, the gene encoding the only known (but still putative) G protein-coupled receptor of Arabidopsis thaliana, is modulated during the cell cycle and during plant development. Overexpression of GCR1 in tobacco (Nicotiana tabacum) BY-2 cells caused an increase in thymidine incorporation and in the mitotic index of aphidicolin synchronized cells. Overexpression of GCR1 in Arabidopsis caused two remarkable phenotypes: seed dormancy was abolished and time to flowering was reduced. Molecular markers of these two developmental processes (phosphatase PP2A and MYB65 in germination; LFY during flowering) were up-regulated in GCR1 overexpressors. These data are consistent with the hypothesis that GCR1 may be a regulator of the cell cycle and that this regulation underlies the developmental changes observed in the GCR1 transformants.

MeSH Terms
Arabidopsis/genetics,growth & development Arabidopsis Proteins Cell Cycle DNA/biosynthesis Gene Expression Regulation, Developmental Germination/genetics Mitotic Index Receptors, Cell Surface/genetics,physiology Receptors, G-Protein-Coupled
Chemicals
Arabidopsis Proteins GCR1 protein, Arabidopsis Receptors, Cell Surface Receptors, G-Protein-Coupled DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Colucci Gabriella
Arena Pharmaceuticals, 6166 Nancy Ridge Drive, San Diego, CA 92121, USA. gcolucci@arenapharm.com
Apone Fabio
Alyeshmerni Nicole
Chalmers Derek
Chrispeels Maarten J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.072087699
Email
gcolucci@arenapharm.com
Published
2002-04-02
Pages
4736-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC123717
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.072087699 PMC Full Text View journal details
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