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PMID: 12376626 Published · ppublish English Journal Article

Expression profiling of the whole Arabidopsis shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction.

Plant physiology ·Vol. 130 ·No. 2 ·2002-10-00 ·Pages 577-90

Charrier B, Champion A, Henry Y, Kreis M

Abstract

The recent publication of the complete sequence of the Arabidopsis genome allowed us to identify and characterize the last two members of the SHAGGY-like kinase (AtSK) gene family. As a result, the study of the overall spatio-temporal organization of the whole AtSK family in Arabidopsis has become an achievable and necessary aim to understand the role of each SHAGGY-like kinase during plant development. An analysis of the transcript level of the 10 members of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction. Transcript levels in several organs, under different growth conditions, were analyzed. To calibrate the results obtained, a number of other genes, such as those coding for the two MAP3Kepsilons and the two MAP4Kalphas, as well as the stress response marker RD29A; the small subunit of the Rubisco photosynthetic enzyme Ats1A; the MEDEA chromatin remodeling factor; and the SCARECROW, ASYMMETRIC LEAVES 1, and SUPERMAN transcription factors all involved in key steps of plant development were used. The analysis of our data revealed that eight of the 10 genes of the AtSK family displayed a pseudo-constitutive expression pattern at the organ level. Conversely, AtSK13 responded to osmotic changes and saline treatment, whereas AtSK31 was flower specific and responded to osmotic changes and darkness.

MeSH Terms
Adaptation, Physiological/drug effects,genetics,radiation effects Arabidopsis/enzymology,genetics,growth & development Arabidopsis Proteins/drug effects,genetics,radiation effects Gene Expression Profiling/methods Gene Expression Regulation, Developmental/drug effects,radiation effects Gene Expression Regulation, Enzymologic/drug effects,radiation effects Gene Expression Regulation, Plant/drug effects,radiation effects Glycogen Synthase Kinase 3/analogs & derivatives,drug effects,genetics,radiation effects Light Osmotic Pressure/drug effects Phylogeny Polyethylene Glycols/pharmacology Reverse Transcriptase Polymerase Chain Reaction/methods Sodium Chloride/pharmacology Stress, Mechanical Transcription Factors/genetics Water/pharmacology,physiology
Chemicals
Arabidopsis Proteins Transcription Factors Water Polyethylene Glycols Sodium Chloride ATSK11 protein, Arabidopsis Glycogen Synthase Kinase 3
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Charrier Bénédicte
Laboratoire de Biologie du Développement des Plantes, Bâtiment 630, Unité Mixte de Recherche-Centre National de la Recherche Scientifique 8618, Université Paris-Sud (XI), 91405 Orsay cedex, France. charrier@ibp.u-psud.fr
Champion Anthony
Henry Yves
Kreis Martin
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.009175
Email
charrier@ibp.u-psud.fr
Published
2002-10-00
Pages
577-90
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC166588
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.009175 PMC Full Text View journal details
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