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PMID: 24272625 Published · ppublish English Journal Article

Four genes in two diverged subfamilies encode the ribulose-1,5-bisphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana.

Plant molecular biology ·Vol. 11 ·No. 6 ·1988-11-00 ·Pages 745-59

Krebbers E, Seurinck J, Herdies L, Cashmore AR, Timko MP

Abstract

The multigene family encoding the small subunit polypeptides of ribulose-1,5-bisphosphate carboxylase/oxygenase in the crucifer Arabidopsis thaliana has been isolated and the organization and structure of the individual members determined. The family consists of four genes which have been divided into two subfamilies on the basis of linkage and DNA and amino acid sequence similarities. Three of the genes, designated ats1B, ats2B, and ats3B, reside in tandem on an 8 kb stretch of the chromosome. These genes share greater than 95% similarity in DNA sequence and encode polypeptides identical in length and 96.7% similar in amino acid sequence. The fourth gene, ats1A, is at least 10 kb removed from, or completely unlinked to the B subfamily. The B subfamily genes are more similar to each other than to ats1A in nucleotide and amino acid sequence. All four genes are interupted by two introns whose placement within the coding region of the genes is conserved. The introns of the B subfamily genes are similar in length and nucleotide sequence, but show no similarity to the introns of ats1A. Comparison of the DNA sequences within the immediate 5' and 3' flanking sequences among the genes revealed only limited regions of homology. S1 analysis shows that all four genes are expressed.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Krebbers E
Plant Genetic Systems, J. Plateaustraat 22, B-9000, Gent, Belgium.
Seurinck J
Herdies L
Cashmore A R
Timko M P
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019515
Published
1988-11-00
Pages
745-59
Language
English
Region
Netherlands
NLM ID
9106343
External Links
PubMed source DOI: 10.1007/BF00019515 View journal details
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