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PMID: 16592473 Published · ppublish English Journal Article

Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5487-91

Coen DM, Bedbrook JR, Bogorad L, Rich A

Abstract

In vitro linked transcription-translation of chloroplast DNA has been used to show that the large subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39] is encoded by Zea mays chloroplast DNA. A BamHI-generated chloroplast DNA sequence cloned in Escherichia coli is shown to direct the in vitro synthesis of this protein identified as large subunit by its size, serological properties, and limited proteolytic digestion products.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Coen D M
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Bedbrook J R
Bogorad L
Rich A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5487
Published
1977-12-00
Pages
5487-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431774
External Links
PubMed source DOI: 10.1073/pnas.74.12.5487 PMC Full Text View journal details
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