Home LiteratureArticle Details
PMID: 15126388 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae SSD1-V confers longevity by a Sir2p-independent mechanism.

Genetics ·Vol. 166 ·No. 4 ·2004-04-00 ·Pages 1661-72

Kaeberlein M, Andalis AA, Liszt GB, Fink GR, Guarente L

Abstract

The SSD1 gene of Saccharomyces cerevisiae is a polymorphic locus that affects diverse cellular processes including cell integrity, cell cycle progression, and growth at high temperature. We show here that the SSD1-V allele is necessary for cells to achieve extremely long life span. Furthermore, addition of SSD1-V to cells can increase longevity independently of SIR2, although SIR2 is necessary for SSD1-V cells to attain maximal life span. Past studies of yeast aging have been performed in short-lived ssd1-d strain backgrounds. We propose that SSD1-V defines a previously undescribed pathway affecting cellular longevity and suggest that future studies on longevity-promoting genes should be carried out in long-lived SSD1-V strains.

MeSH Terms
Alleles Gene Expression Regulation, Fungal Genes, Fungal/genetics Histone Deacetylases/metabolism Longevity/genetics Oligonucleotide Array Sequence Analysis Osmolar Concentration Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/metabolism Silent Information Regulator Proteins, Saccharomyces cerevisiae/metabolism Sirtuin 2 Sirtuins/metabolism Temperature Transformation, Genetic
Chemicals
Saccharomyces cerevisiae Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae Ssd1 protein, S cerevisiae SIR2 protein, S cerevisiae Sirtuin 2 Sirtuins Histone Deacetylases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kaeberlein Matt
Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA. kaeber@u.washington.edu
Andalis Alex A
Liszt Gregory B
Fink Gerald R
Guarente Leonard
References (56)
56 references, click to expand
  1. Multicopy suppressors of the sly1 temperature-sensitive mutation in the ER-Golgi vesicular transport in Saccharomyces cerevisiae.
    Yeast. 2001 Aug;18(11):1003-14 PMID: 11481671
  2. Regulation of tubulin polypeptides and microtubule function: Luv1p [correction of Rki1p] interacts with the beta-tubulin binding protein Rbl2p.
    Chromosoma. 1998 Dec;107(6-7):471-8 PMID: 9914379
  3. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity.
    Genetics. 2002 Jan;160(1):83-95 PMID: 11805047
  4. Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae.
    Mol Biol Cell. 2002 Feb;13(2):503-14 PMID: 11854408
  5. Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration.
    Nature. 2002 Jul 18;418(6895):344-8 PMID: 12124627
  6. ARL1 and membrane traffic in Saccharomyces cerevisiae.
    Yeast. 2002 Sep 15;19(12):1039-56 PMID: 12210899
  7. High osmolarity extends life span in Saccharomyces cerevisiae by a mechanism related to calorie restriction.
    Mol Cell Biol. 2002 Nov;22(22):8056-66 PMID: 12391171
  8. Mutational analysis reveals a role for the C terminus of the proteasome subunit Rpt4p in spindle pole body duplication in Saccharomyces cerevisiae.
    Genetics. 2002 Oct;162(2):705-20 PMID: 12399382
  9. A Saccharomyces cerevisiae mutant with increased virulence.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2766-70 PMID: 12589024
  10. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing.
    Genetics. 2003 Jul;164(3):895-907 PMID: 12871902
  11. Cloning and characterization of four SIR genes of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Feb;6(2):688-702 PMID: 3023863
  12. Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae.
    Genetics. 1987 May;116(1):9-22 PMID: 3297920
  13. A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA.
    Cell. 1989 Mar 10;56(5):771-6 PMID: 2647300
  14. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
    Cell. 1990 Nov 16;63(4):751-62 PMID: 2225075
  15. Micromanipulation and dissection of asci.
    Methods Enzymol. 1991;194:21-37 PMID: 2005789
  16. The SIT4 protein phosphatase functions in late G1 for progression into S phase.
    Mol Cell Biol. 1991 Apr;11(4):2133-48 PMID: 1848673
  17. The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function.
    Mol Cell Biol. 1991 Jun;11(6):3369-73 PMID: 1645449
  18. A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth.
    Mol Cell Biol. 1992 Mar;12(3):1162-78 PMID: 1545797
  19. Improved method for high efficiency transformation of intact yeast cells.
    Nucleic Acids Res. 1992 Mar 25;20(6):1425 PMID: 1561104
  20. A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae.
    Mol Gen Genet. 1993 May;239(1-2):169-76 PMID: 8510644
  21. Yeast G1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation.
    EMBO J. 1993 Dec 15;12(13):5277-86 PMID: 8262070
  22. Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation.
    J Cell Biol. 1994 Dec;127(5):1381-94 PMID: 7962097
  23. Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae.
    Cell. 1995 Feb 10;80(3):485-96 PMID: 7859289
  24. Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (Slt2) mitogen-activated protein kinase pathway.
    Mol Cell Biol. 1995 Oct;15(10):5740-9 PMID: 7565726
  25. NCA3, a nuclear gene involved in the mitochondrial expression of subunits 6 and 8 of the Fo-F1 ATP synthase of S. cerevisiae.
    Curr Genet. 1995 Apr;27(5):409-16 PMID: 7586026
  26. LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth.
    Genetics. 1995 Nov;141(3):857-71 PMID: 8582632
  27. Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae.
    Mol Cell Biol. 1996 Jul;16(7):3255-63 PMID: 8668140
  28. Coordinated regulation of gene expression by the cell cycle transcription factor Swi4 and the protein kinase C MAP kinase pathway for yeast cell integrity.
    EMBO J. 1996 Sep 16;15(18):5001-13 PMID: 8890173
  29. An unusual form of transcriptional silencing in yeast ribosomal DNA.
    Genes Dev. 1997 Jan 15;11(2):241-54 PMID: 9009206
  30. Transcriptional silencing of Ty1 elements in the RDN1 locus of yeast.
    Genes Dev. 1997 Jan 15;11(2):255-69 PMID: 9009207
  31. SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway.
    Science. 1997 Mar 21;275(5307):1781-4 PMID: 9065400
  32. Mutations in the Saccharomyces cerevisiae type 2A protein phosphatase catalytic subunit reveal roles in cell wall integrity, actin cytoskeleton organization and mitosis.
    Genetics. 1997 Feb;145(2):227-41 PMID: 9071579
  33. Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae.
    Cell. 1997 May 2;89(3):381-91 PMID: 9150138
  34. Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest.
    Mol Cell Biol. 1997 Jun;17(6):3429-39 PMID: 9154842
  35. Ssd1p of Saccharomyces cerevisiae associates with RNA.
    J Biol Chem. 1997 Jun 27;272(26):16103-9 PMID: 9195905
  36. Extrachromosomal rDNA circles--a cause of aging in yeast.
    Cell. 1997 Dec 26;91(7):1033-42 PMID: 9428525
  37. A screen for upstream components of the yeast protein kinase C signal transduction pathway identifies the product of the SLG1 gene.
    Mol Gen Genet. 1998 Apr;258(1-2):148-55 PMID: 9613583
  38. Effects of mutations in DNA repair genes on formation of ribosomal DNA circles and life span in Saccharomyces cerevisiae.
    Mol Cell Biol. 1999 May;19(5):3848-56 PMID: 10207108
  39. Elimination of replication block protein Fob1 extends the life span of yeast mother cells.
    Mol Cell. 1999 Apr;3(4):447-55 PMID: 10230397
  40. Analysis of genetic interactions between DHH1, SSD1 and ELM1 indicates their involvement in cellular morphology determination in Saccharomyces cerevisiae.
    Yeast. 1999 Apr;15(6):481-96 PMID: 10234786
  41. A conditional U5 snRNA mutation affecting pre-mRNA splicing and nuclear pre-mRNA retention identifies SSD1/SRK1 as a general splicing mutant suppressor.
    Nucleic Acids Res. 1999 Sep 1;27(17):3455-65 PMID: 10446233
  42. Genetic and physical interactions involving the yeast nuclear cap-binding complex.
    Mol Cell Biol. 1999 Oct;19(10):6543-53 PMID: 10490594
  43. The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms.
    Genes Dev. 1999 Oct 1;13(19):2570-80 PMID: 10521401
  44. The short life span of Saccharomyces cerevisiae sgs1 and srs2 mutants is a composite of normal aging processes and mitotic arrest due to defective recombination.
    Genetics. 2001 Apr;157(4):1531-42 PMID: 11290710
  45. Functional analysis of RRD1 (YIL153w) and RRD2 (YPL152w), which encode two putative activators of the phosphotyrosyl phosphatase activity of PP2A in Saccharomyces cerevisiae.
    Mol Gen Genet. 2000 Jan;262(6):1081-92 PMID: 10660069
  46. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.
    Nature. 2000 Feb 17;403(6771):795-800 PMID: 10693811
  47. The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases.
    Proc Natl Acad Sci U S A. 2000 May 23;97(11):5807-11 PMID: 10811920
  48. A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.
    Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6658-63 PMID: 10841563
  49. Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae.
    Science. 2000 Sep 22;289(5487):2126-8 PMID: 11000115
  50. A role for Sds3p, a component of the Rpd3p/Sin3p deacetylase complex, in maintaining cellular integrity in Saccharomyces cerevisiae.
    Mol Genet Genomics. 2001 May;265(3):560-8 PMID: 11405640
  51. Diagnosis and management of delirium near the end of life.
    Ann Intern Med. 2001 Jul 3;135(1):32-40 PMID: 11434730
  52. Sif2p interacts with Sir4p amino-terminal domain and antagonizes telomeric silencing in yeast.
    Curr Biol. 1998 Jun 18;8(13):787-90 PMID: 9651685
  53. Genetic interaction between YPT6 and YPT1 in Saccharomyces cerevisiae.
    Yeast. 1998 Jul;14(10):915-22 PMID: 9717237
  54. Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains.
    Genetics. 1998 Dec;150(4):1443-57 PMID: 9832522
  55. A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling.
    Mol Cell Biol. 1999 Feb;19(2):1056-67 PMID: 9891041
  56. A genome-wide scan for linkage to human exceptional longevity identifies a locus on chromosome 4.
    Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10505-8 PMID: 11526246
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.166.4.1661
Email
kaeber@u.washington.edu
Published
2004-04-00
Pages
1661-72
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1470832
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.166.4.1661 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2