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PMID: 8582632 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth.

Genetics ·Vol. 141 ·No. 3 ·1995-11-00 ·Pages 857-71

Doseff AI, Arndt KT

Abstract

Saccharomyces cerevisiae mutations that cause a requirement for SSD1-v for viability were isolated, yielding one new gene, LAS1, and three previously identified genes, SIT4, BCK1/SLK1, and SMP3. Three of these genes, LAS1, SIT4, and BCK1/SLK1, encode proteins that have roles in bud formation or morphogenesis. LAS1 is essential and loss of LAS1 function causes the cells to arrest as 80% unbudded cells and 20% large budded cells that accumulate many vesicles at the mother-daughter neck. Overexpression of LAS1 results in extra cell surface projections in the mother cell, alterations in actin and SPA2 localization, and the accumulation of electron-dense structures along the periphery of both the mother cell and the bud. The nuclear localization of LAS1 suggests a role of LAS1 for regulating bud formation and morphogenesis via the expression of components that function directly in these processes.

MeSH Terms
Amino Acid Sequence Cell Size Cell Wall/metabolism Fungal Proteins/genetics,physiology Genes, Fungal Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Morphogenesis Nuclear Proteins/genetics,physiology Phosphoprotein Phosphatases/genetics,physiology Protein Kinases/genetics,physiology Protein Phosphatase 2 Recombinant Fusion Proteins/biosynthesis Saccharomyces cerevisiae/cytology,genetics,growth & development Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins LAS1 protein, S cerevisiae Nuclear Proteins Recombinant Fusion Proteins SMP3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Protein Kinases BCK1 protein, S cerevisiae Mitogen-Activated Protein Kinase Kinases Phosphoprotein Phosphatases Protein Phosphatase 2 SIT4 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Doseff A I
Cold Spring Harbor Laboratory, New York 11724-2212, USA.
Arndt K T
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/141.3.857
Published
1995-11-00
Pages
857-71
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206850
Subset
IM
Grants
NIGMS NIH HHS · GM-45179 · United States
Databases
GENBANK
U09670
External Links
PubMed source DOI: 10.1093/genetics/141.3.857 PMC Full Text View journal details
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