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PMID: 8262070 Published · ppublish English Journal Article

Yeast G1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation.

The EMBO journal ·Vol. 12 ·No. 13 ·1993-12-15 ·Pages 5277-86

Cvrcková F, Nasmyth K

Abstract

Cyclin-dependent protein kinases have a central role in cell cycle regulation. In Saccharomyces cerevisiae, Cdc28 kinase and the G1 cyclins Cln1, 2 and 3 are required for DNA replication, duplication of the spindle pole body and bud emergence. These three independent processes occur simultaneously in late G1 when the cells reach a critical size, an event known as Start. At least one of the three Clns is necessary for Start. Cln3 is believed to activate Cln1 and Cln2, which can then stimulate their own accumulation by means of a positive feedback loop. They (or Cln3) also activate another pair of cyclins, Clb5 and 6, involved in initiating S phase. Little is known about the role of Clns in spindle pole body duplication and budding. We report here the isolation of a gene (CLA2/BUD2/ERC25) that codes for a homologue of mammalian Ras-associated GTPase-activating proteins (GAPs) and is necessary for budding only in cln1 cln2 cells. This suggests that Cln1 and Cln2 may have a direct role in bud formation.

Related Genes
CLA2 GAP IRA1 IRA2 NF1
MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Cloning, Molecular Cyclins/physiology Fungal Proteins/physiology GTP Phosphohydrolase Activators GTP Phosphohydrolases GTPase-Activating Proteins Genes, Lethal Humans Infant, Newborn Molecular Sequence Data Proteins/physiology Saccharomyces cerevisiae/cytology Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Tripeptidyl-Peptidase 1 ras GTPase-Activating Proteins
Chemicals
BUD2 protein, S cerevisiae CLN1 protein, S cerevisiae CLN2 protein, S cerevisiae Cyclins Fungal Proteins GTP Phosphohydrolase Activators GTPase-Activating Proteins Proteins Saccharomyces cerevisiae Proteins Tripeptidyl-Peptidase 1 ras GTPase-Activating Proteins TPP1 protein, human GTP Phosphohydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cvrcková F
IMP, Wien, Austria.
Nasmyth K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-12-15
Pages
5277-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413793
Subset
IM
Databases
GENBANK
X74130
External Links
PubMed source PMC Full Text View journal details
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