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PMID: 15116071 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ssn6-Tup1 requires the ISW2 complex to position nucleosomes in Saccharomyces cerevisiae.

The EMBO journal ·Vol. 23 ·No. 11 ·2004-06-02 ·Pages 2246-57

Zhang Z, Reese JC

Abstract

The Imitation SWItch (ISWI) chromatin remodeling factors have been implicated in nucleosome positioning. In vitro, they can mobilize nucleosomes bi-directionally, making it difficult to envision how they can establish precise translational positioning of nucleosomes in vivo. It has been proposed that they require other cellular factors to do so, but none has been identified thus far. Here, we demonstrate that both ISW2 and TUP1 are required to position nucleosomes across the entire coding sequence of the DNA damage-inducible gene RNR3. The chromatin structure downstream of the URS is indistinguishable in Deltaisw2 and Deltatup1 mutants, and the crosslinking of Tup1 and Isw2 to RNR3 is independent of each other, indicating that both complexes are required to maintain repressive chromatin structure. Furthermore, Tup1 repressed RNR3 and blocked preinitiation complex formation in the Deltaisw2 mutant, even though nucleosome positioning was completely disrupted over the promoter and ORF. Our study has revealed a novel collaboration between two nucleosome-positioning activities in vivo, and suggests that disruption of nucleosome positioning is insufficient to cause a high level of transcription.

MeSH Terms
Adenosine Triphosphatases/metabolism Cell Nucleus/metabolism Chromatin/metabolism Chromatin Immunoprecipitation DNA-Binding Proteins/metabolism Nuclear Proteins/metabolism Nucleosomes/metabolism Promoter Regions, Genetic Repressor Proteins/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/metabolism Transcription Factors/metabolism
Chemicals
CYC8 protein, S cerevisiae Chromatin DNA-Binding Proteins ISWI protein Nuclear Proteins Nucleosomes Repressor Proteins Saccharomyces cerevisiae Proteins TUP1 protein, S cerevisiae Transcription Factors Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhang Zhengjian
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Reese Joseph C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600227
Published
2004-06-02
Epub
2004-00-29
Pages
2246-57
Language
English
Region
England
NLM ID
8208664
PMCID
PMC419907
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058672 · United States
NIGMS NIH HHS · GM58672 · United States
External Links
PubMed source DOI: 10.1038/sj.emboj.7600227 PMC Full Text View journal details
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