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PMID: 15024052 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo.

Molecular and cellular biology ·Vol. 24 ·No. 7 ·2004-04-00 ·Pages 2605-13

McConnell AD, Gelbart ME, Tsukiyama T

Abstract

We report the identification of two new subunits of the Isw2 chromatin-remodeling complex in Saccharomyces cerevisiae. Both proteins, Dpb4p and Yjl065cp (named Dls1p), contain histone fold motifs and are homologous to the two smallest subunits of ISWI-containing CHRAC complexes in higher eukaryotes. Dpb4p is also a subunit of the DNA polymerase epsilon (polepsilon) complex, and Dls1p is homologous to another polepsilon subunit, Dpb3p. Therefore, these small histone fold proteins may fulfill functions that are required for both polepsilon and Isw2 complexes. We characterized the role of Dls1p in known roles of the Isw2 complex in vivo. Transcriptional analyses reveal that the Isw2 complex requires Dls1p to various degrees at a wide variety of loci in vivo. Consistent with this, Dls1p is required for Isw2-dependent chromatin remodeling in vivo, although the requirement for this protein varies among Isw2 targets. Dls1p is likely required for functions of the Isw2 complex at steps subsequent to its interaction with chromatin, since a dls1 mutation does not affect cross-linking of Isw2 with chromatin.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Animals Chromatin/metabolism DNA-Binding Proteins/genetics,metabolism Gene Expression Profiling Gene Expression Regulation, Fungal Histones/metabolism Macromolecular Substances Nuclear Proteins/metabolism Oligonucleotide Array Sequence Analysis Protein Subunits/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism Transcription Factors/genetics,metabolism
Chemicals
Chromatin DLS1 protein, S cerevisiae DNA-Binding Proteins Histones ISWI protein Macromolecular Substances Nuclear Proteins Protein Subunits Saccharomyces cerevisiae Proteins Transcription Factors Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McConnell Audrey D
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Gelbart Marnie E
Tsukiyama Toshio
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.7.2605-2613.2004
Published
2004-04-00
Pages
2605-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC371119
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058465 · United States
NIGMS NIH HHS · GM58465 · United States
External Links
PubMed source DOI: 10.1128/MCB.24.7.2605-2613.2004 PMC Full Text View journal details
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