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PMID: 1459965 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Presence of methylated adenine in GATC sequences in chromosomal DNAs from Campylobacter species.

Journal of bacteriology ·Vol. 174 ·No. 24 ·1992-12-00 ·Pages 8156-7

Edmonds P, Hall BM, Edwards WR, Hartline KM

Abstract

We digested chromosomal DNAs from 12 Campylobacter strains (C. jejuni, 4 strains; C. coli, 2 strains; C. fetus subsp. fetus, 2 strains; C. hyointestinalis, 2 strains; and C. upsaliensis, 2 strains) and from 4 Helicobacter strains (H. pylori, 2 strains; and H. mustelae, 2 strains) with HindIII, SstI, BamHI, DpnI, MboI, and Sau3AI. Restriction fragments were then separated by electrophoresis in 1% agarose or 10% polyacrylamide gels. Only DNAs from three Campylobacter species (C. jejuni, C. coli, and C. upsaliensis) were digested with DpnI (an enzyme that recognizes only methylated adenine in GATC sequences). We used MboI and Sau3AI to confirm these findings.

MeSH Terms
Adenine/metabolism Base Composition Campylobacter/genetics Chromosomes, Bacterial/metabolism DNA, Bacterial/metabolism Methylation
Chemicals
DNA, Bacterial Adenine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Edmonds P
School of Biology, Georgia Institute of Technology, Atlanta 30332.
Hall B M
Edwards W R
Hartline K M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.24.8156-8157.1992
Published
1992-12-00
Pages
8156-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207557
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.24.8156-8157.1992 PMC Full Text View journal details
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