Home LiteratureArticle Details
PMID: 6094478 Published · ppublish English Comparative Study Journal Article

DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage.

Journal of bacteriology ·Vol. 160 ·No. 2 ·1984-11-00 ·Pages 586-90

Barbeyron T, Kean K, Forterre P

Abstract

We have examined the presence of methylated adenine at GATC sequences (Dam phenotype) in the DNA of 23 eubacteria and 13 archaebacteria by using isoshizomer restriction enzymes. We have found a completely Dam+ phenotype in bacteria of nine genera related to the families Enterobacteriaceae, Parvobacteriaceae, and Vibrionaceae, and in the five cyanobacteria tested. We have found a partial Dam+ phenotype in the two archaebacteria Halobacterium saccharovorum and Methanobacterium sp. strain Ivanov. All of the other archaebacteria (three genera) and eubacteria (nine genera) tested were Dam-. Phylogenetic analysis, based on the evolutionary tree of Fox et al. (Science 209:457-463, 1980), indicates that dam methylation in the Escherichia coli lineage appeared recently in bacterial evolution and is restricted to a small range of closely related bacteria.

MeSH Terms
Adenine/analogs & derivatives Archaea/genetics Bacteria/genetics Base Sequence DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Gram-Negative Bacteria/genetics Methylation Phylogeny Species Specificity
Chemicals
DNA, Bacterial DNA Restriction Enzymes Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barbeyron T
Kean K
Forterre P
References (28)
28 references, click to expand
  1. The phylogeny of prokaryotes.
    Science. 1980 Jul 25;209(4455):457-63 PMID: 6771870
  2. A general method for the purification of restriction enzymes.
    Nucleic Acids Res. 1978 Jul;5(7):2373-80 PMID: 673857
  3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.
    J Mol Biol. 1977 Jul;114(1):153-68 PMID: 20509
  4. Absence in Bacillus subtilis and Staphylococcus aureus of the sequence-specific deoxyribonucleic acid methylation that is conferred in Escherichia coli K-12 by the dam and dcm enzymes.
    J Bacteriol. 1981 Jul;147(1):259-61 PMID: 6263867
  5. 5-methylcytosine and 6-methylamino-purine in bacterial DNA.
    Nature. 1968 Jun 15;218(5146):1066-7 PMID: 5656625
  6. Cytosine methylation of the sequence GATC in a mycoplasma.
    J Bacteriol. 1982 Sep;151(3):1420-4 PMID: 6286600
  7. Deoxyribonucleic acid adenine and cytosine methylation in Salmonella typhimurium and Salmonella typhi.
    J Bacteriol. 1979 Nov;140(2):574-9 PMID: 387741
  8. A restriction endonuclease from Staphylococcus aureus.
    Nucleic Acids Res. 1976 Nov;3(11):3193-202 PMID: 1005115
  9. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  10. Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature.
    Arch Mikrobiol. 1972;84(1):54-68 PMID: 4559703
  11. Evolutionary relationships in vibrio and Photobacterium: a basis for a natural classification.
    Annu Rev Microbiol. 1983;37:369-98 PMID: 6357056
  12. A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.
    Nucleic Acids Res. 1978 Jun;5(6):1729-39 PMID: 673834
  13. The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
    Nucleic Acids Res. 1983 Feb 11;11(3):837-51 PMID: 6300769
  14. Single-strand and double-strand cleavage at half-modified and fully modified recognition sites for the restriction nucleases Sau3a and Taqi.
    Gene. 1980 Dec;12(3-4):267-75 PMID: 6265320
  15. Replicational fidelity: mechanisms of mutation avoidance and mutation fixation.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:937-46 PMID: 385236
  16. Chromosomal replication origin from the marine bacterium Vibrio harveyi functions in Escherichia coli: oriC consensus sequence.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1164-8 PMID: 6338499
  17. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.
    Mol Gen Genet. 1978 Jul 25;163(3):307-12 PMID: 355857
  18. Methyl-directed repair of DNA base-pair mismatches in vitro.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4639-43 PMID: 6308634
  19. Studies on the biological role of DNA methylation: V. The pattern of E.coli DNA methylation.
    Nucleic Acids Res. 1982 Nov 25;10(22):7247-59 PMID: 6296768
  20. Halobacterium halobium phage øH.
    EMBO J. 1982;1(1):87-92 PMID: 16453410
  21. DNA methylation and its possible biological roles.
    Prog Nucleic Acid Res Mol Biol. 1981;25:33-52 PMID: 6261297
  22. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12.
    J Mol Biol. 1974 May 15;85(2):309-22 PMID: 4600143
  23. Genetic recombination: strand transfer and mismatch repair.
    Annu Rev Biochem. 1978;47:847-80 PMID: 150254
  24. Excision and repair of mismatched base pairs in transformation of Streptococcus pneumoniae.
    Mol Gen Genet. 1980 Apr;178(1):191-201 PMID: 6929947
  25. Two sequence-specific endonucleases from Moraxella bovis.
    J Mol Biol. 1977 Jul;114(1):169-79 PMID: 909085
  26. Effect of some metal ions on the growth of Halobacterium cutirubrum.
    Can J Microbiol. 1960 Apr;6:165-9 PMID: 14444586
  27. Identification of base mismatches recognized by the heteroduplex-DNA-repair system of Streptococcus pneumoniae.
    Cell. 1982 Dec;31(2 Pt 1):327-36 PMID: 6297760
  28. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1983 Jan 11;11(1):r135-67 PMID: 6306557
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.2.586-590.1984
Published
1984-11-00
Pages
586-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214774
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.160.2.586-590.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2