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PMID: 1657894 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Presence of 5-methylcytosine in CC(A/T)GG sequences (Dcm methylation) in DNAs from different bacteria.

Journal of bacteriology ·Vol. 173 ·No. 23 ·1991-12-00 ·Pages 7692-4

Gomez-Eichelmann MC, Levy-Mustri A, Ramirez-Santos J

Abstract

The presence of CC(A/T)GG sequences with methylated internal cytosine (Dcm methylation) was determined in DNA from different genera of eubacteria. This methylation was studied by using restriction enzymes EcoRII and BstNI, which cleave unmethylated or methylated CC(A/T)GG sequences. Dcm methylation was only detected in genera of the family Enterobacteriaceae closely related to Escherichia: Shigella, Citrobacter, Salmonella, and Klebsiella.

MeSH Terms
5-Methylcytosine Bacteria/chemistry Base Sequence Cytosine/analogs & derivatives,analysis DNA Restriction Enzymes DNA, Bacterial/chemistry,isolation & purification Electrophoresis, Polyacrylamide Gel Oligodeoxyribonucleotides/chemistry,isolation & purification
Chemicals
DNA, Bacterial Oligodeoxyribonucleotides 5-Methylcytosine Cytosine DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gomez-Eichelmann M C
Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City.
Levy-Mustri A
Ramirez-Santos J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.23.7692-7694.1991
Published
1991-12-00
Pages
7692-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212538
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.23.7692-7694.1991 PMC Full Text View journal details
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