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PMID: 2066345 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The hemimethylated replication origin of Escherichia coli can be initiated in vitro.

Journal of bacteriology ·Vol. 173 ·No. 14 ·1991-07-00 ·Pages 4537-9

Boye E

Abstract

Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.

MeSH Terms
Bacterial Proteins/metabolism DNA Replication DNA, Bacterial/genetics DNA-Binding Proteins Escherichia coli/genetics Kinetics Methylation Plasmids Restriction Mapping
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins DnaA protein, Bacteria
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Boye E
Department of Biophysics, Norwegian Radium Hospital, Oslo.
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.14.4537-4539.1991
Published
1991-07-00
Pages
4537-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208120
Subset
IM
Grants
NIGMS NIH HHS · GM39582 · United States
External Links
PubMed source DOI: 10.1128/jb.173.14.4537-4539.1991 PMC Full Text View journal details
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