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PMID: 1400176 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli.

Journal of bacteriology ·Vol. 174 ·No. 19 ·1992-10-00 ·Pages 6256-63

Shi W, Zhou Y, Wild J, Adler J, Gross CA

Abstract

The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli. Cells deleted for dnaK or dnaJ, or with some mutations in the dnaK or grpE gene, are nonmotile, lack flagella, exhibit a 10- to 20-fold decrease in the rate of synthesis of flagellin, and show reduced rates of transcription of both the flhD master operon (encoding FlhD and FlhC) and the fliA operon (encoding sigma F). Genetic studies suggest that DnaK and DnaJ define a regulatory pathway affecting flhD and fliA synthesis that is independent of cyclic AMP-catabolite gene activator protein or the chemotaxis system.

Related Genes
DnaJ DnaK GrpE
MeSH Terms
Bacterial Proteins/genetics Cell Movement/genetics Escherichia coli/genetics,metabolism,ultrastructure Escherichia coli Proteins Flagella/physiology,ultrastructure Flagellin/biosynthesis Genes, Regulator HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins/genetics Mutation Recombinant Fusion Proteins/biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins DnaJ protein, E coli Escherichia coli Proteins GrpE protein, Bacteria GrpE protein, E coli HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins Recombinant Fusion Proteins Flagellin dnaK protein, E coli
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shi W
Department of Biochemistry, University of Wisconsin, Madison 53706.
Zhou Y
Wild J
Adler J
Gross C A
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.19.6256-6263.1992
Published
1992-10-00
Pages
6256-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207695
Subset
IM
Grants
NIGMS NIH HHS · GM36278 · United States
External Links
PubMed source DOI: 10.1128/jb.174.19.6256-6263.1992 PMC Full Text View journal details
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