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PMID: 2832369 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5' consensus sequences of operons under FlbB and FlaI control.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1575-81

Bartlett DH, Frantz BB, Matsumura P

Abstract

The regulation of the expression of the operons in the flagella-chemotaxis regulon in Escherichia coli has been shown to be a highly ordered cascade which closely parallels the assembly of the flagellar structure and the chemotaxis machinery (T. Iino, Annu. Rev. Genet. 11:161-182, 1977; Y. Komeda, J. Bacteriol. 168: 1315-1318). The master operon, flbB, has been sequenced, and one of its gene products (FlaI) has been identified. On the basis of the deduced amino acid sequence, the FlbB protein has similarity to an alternate sigma factor which is responsible for expression of flagella in Bacillus subtilis. In addition, we have sequenced the 5' regions of a number of flagellar operons and compared these sequences with the 5' region of flagellar operons directly and indirectly under FlbB and FlaI control. We found both a consensus sequence which has been shown to be in all other flagellar operons (J. D. Helmann and M. J. Chamberlin, Proc. Natl. Acad. Sci. USA 84:6422-6424) and a derivative consensus sequence, which is found only in the 5' region of operons directly under FlbB and FlaI control.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics,ultrastructure Flagella/physiology Gene Expression Regulation Genes, Bacterial Genetic Vectors Molecular Sequence Data Operon Plasmids Sequence Homology, Nucleic Acid Transcription Factors/genetics Transcription, Genetic
Chemicals
Transcription Factors DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bartlett D H
Department of Biological Sciences, University of Illinois, Chicago 60680.
Frantz B B
Matsumura P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1575-1581.1988
Published
1988-04-00
Pages
1575-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211004
Subset
IM
Grants
NIAID NIH HHS · AI 18985 · United States
Databases
GENBANK
M19439
External Links
PubMed source DOI: 10.1128/jb.170.4.1575-1581.1988 PMC Full Text View journal details
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