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PMID: 2563997 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures.

Journal of bacteriology ·Vol. 171 ·No. 3 ·1989-03-00 ·Pages 1379-85

Fayet O, Ziegelhoffer T, Georgopoulos C

Abstract

The products of the groES and groEL genes of Escherichia coli, constituting the groE operon, are known to be required for growth at high temperature (42 degrees C) and are members of the heat shock regulon. Using a genetic approach, we examined the requirement for these gene products for bacterial growth at low temperature (17 to 30 degrees C). To do this, we constructed various groES groEL heterodiploid derivative strains. By inactivating one of the groE operons by a polar insertion, it was shown by bacteriophage P1 transduction that at least one of the groE genes was essential for growth at low temperature. Further P1 transduction experiments with strains that were heterodiploid for only one of the groE genes demonstrated that both groE gene products were required for growth at low temperature, which suggested a fundamental role for the groE proteins in E. coli growth and physiology.

MeSH Terms
Bacterial Proteins/genetics Blotting, Western Chaperonin 10 Chaperonin 60 Escherichia coli/genetics,growth & development Genes Genes, Bacterial Genes, Regulator Genotype Heat-Shock Proteins/genetics Operon Phenotype Temperature Transduction, Genetic
Chemicals
Bacterial Proteins Chaperonin 10 Chaperonin 60 Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fayet O
Centre de Recherche de Biochimie et Genetique Cellulaires, Centre National de Recherche Scientifique, Toulouse, France.
Ziegelhoffer T
Georgopoulos C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.3.1379-1385.1989
Published
1989-03-00
Pages
1379-85
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209756
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.3.1379-1385.1989 PMC Full Text View journal details
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