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PMID: 13679573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 20 ·2003-09-30 ·Pages 11429-34

Shepard KA, Gerber AP, Jambhekar A, Takizawa PA, Brown PO, Herschlag D, DeRisi JL, Vale RD

Abstract

Cytoplasmic mRNA localization provides a means of generating cell asymmetry and segregating protein activity. Previous studies have identified two mRNAs that localize to the bud tips of the yeast Saccharomyces cerevisiae. To identify additional localized mRNAs, we immunoprecipitated the RNA transport components She2p, She3p, and Myo4p and performed DNA microarray analysis of their associated RNAs. A secondary screen, using a GFP-tagged RNA reporter assay, identified 22 mRNAs that are localized to bud tips. These messages encode a wide variety of proteins, including several involved in stress responses and cell wall maintenance. Many of these proteins are asymmetrically localized to buds. However, asymmetric localization also occurs in the absence of RNA transport, suggesting the existence of redundant protein localization mechanisms. In contrast to findings in metazoans, the untranslated regions are dispensable for mRNA localization in yeast. This study reveals an unanticipated widespread use of RNA transport in budding yeast.

MeSH Terms
Biological Transport Cytoplasm/metabolism Oligonucleotide Array Sequence Analysis Precipitin Tests RNA, Messenger/metabolism
Chemicals
RNA, Messenger
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Shepard K A
Department of Cellular and Molecular Pharmacology and Biochemistry and Biophysics and Howard Hughes Medical Institute, University of California, San Francisco, CA 94107, USA.
Gerber A P
Jambhekar A
Takizawa P A
Brown P O
Herschlag D
DeRisi J L
Vale R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.2033246100
Published
2003-09-30
Epub
2003-00-17
Pages
11429-34
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC208774
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038499 · United States
PHS HHS · 38499 · United States
External Links
PubMed source DOI: 10.1073/pnas.2033246100 PMC Full Text View journal details
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