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PMID: 13367 Published · ppublish English Journal Article

Inhibitors of genetic recombination in pneumococci.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 1 ·1977-01-00 ·Pages 296-9

Seto H, Tomasz A

Abstract

Treatment of transformable pneumococci with DNA-intercalating agents shortly after the uptake of DNA molecules inhibited the appearance of genetic transformants. The same drug treatments applied 20 min after DNA uptake were ineffective. Ethidium bromide, proflavin, daunomycin, actinomycin D, and platinum red were found to be effective inhibitors. Donor DNA molecules reisolated from the drug-treated bacteria appeared to be associated with the resident DNA in an abnormal manner, and they had only poor transforming activity.

MeSH Terms
DNA, Bacterial/metabolism Dactinomycin/pharmacology Daunorubicin/pharmacology Ethidium/pharmacology Proflavine/pharmacology Recombination, Genetic/drug effects Streptococcus pneumoniae/drug effects Transformation, Genetic/drug effects
Chemicals
DNA, Bacterial Dactinomycin Proflavine Ethidium Daunorubicin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seto H
Tomasz A
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.1.296
Published
1977-01-00
Pages
296-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393246
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.1.296 PMC Full Text View journal details
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