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PMID: 7544 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci.

Journal of bacteriology ·Vol. 126 ·No. 3 ·1976-06-00 ·Pages 1113-8

Seto H, Tomasz A

Abstract

The conversion of surface-adsorbed deoxyribonucleic acid (DNA) molecules to a state in which they are inaccessible to exogenous deoxyribonuclease requires specifically calcium ions; magnesium ions cannot replace calcium ions. Virtually maximal levels of nuclease-resistant DNA binding and genetic transformation can be obtained in media free from magnesium and containing only calcium ions. It is suggested that the calcium-requiring process is the transport of DNA molecules across the plasma membrane. Magnesium ions stimulate both the loss of surface-adsorbed DNA to the medium and the extracellular degradation of DNA.

MeSH Terms
Calcium/pharmacology Cell Membrane/metabolism DNA, Bacterial/metabolism Deoxyribonucleases/metabolism Edetic Acid/pharmacology Magnesium/pharmacology Streptococcus pneumoniae/enzymology,growth & development,metabolism Transformation, Genetic/drug effects
Chemicals
DNA, Bacterial Edetic Acid Deoxyribonucleases Magnesium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seto H
Tomasz A
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.3.1113-1118.1976
Published
1976-06-00
Pages
1113-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233133
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.3.1113-1118.1976 PMC Full Text View journal details
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