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PMID: 1323953 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of the tet(M) determinant of Tn916: evidence for regulation by transcription attenuation.

Antimicrobial agents and chemotherapy ·Vol. 36 ·No. 4 ·1992-04-00 ·Pages 769-78

Su YA, He P, Clewell DB

Abstract

The nucleotide sequence of the tetracycline resistance determinant tet(M), located on conjugative transposon Tn916 of Enterococcus faecalis, was determined and found to encode a 72,486-dalton protein exhibiting a high degree of homology with other tet(M) determinants. A short open reading frame corresponding to a 28-amino-acid peptide and containing a number of inverted repeat sequences was noted immediately upstream of tet(M), suggesting that regulation might occur by a mechanism involving transcriptional attenuation. Transcription analyses found this to indeed be the case, showing that the expression of tet(M) resulted from an extension of a small transcript representing the upstream leader region into the resistance determinant. Exposure of cells to tetracycline resulted in a significant increase in the amount of tet(M) transcription; this increase could be explained on the basis of increased transcriptional read-through from the upstream transcript. A model suggesting how transcriptional attenuation might operate in this system is presented.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Transposable Elements DNA, Bacterial/analysis Enterococcus faecalis/genetics Escherichia coli/genetics Molecular Sequence Data Tetracycline Resistance/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Su Y A
Department of Biologic Science, School of Dentistry, University of Michigan, Ann Arbor 48109.
He P
Clewell D B
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34 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.36.4.769
Published
1992-04-00
Pages
769-78
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC189400
Subset
IM
Grants
NIAID NIH HHS · AI10318 · United States
Databases
GENBANK
M83279, M83280, M83281, M83282, M83283, M83284, M83285, M85225, X56353, X65253, X65254
External Links
PubMed source DOI: 10.1128/AAC.36.4.769 PMC Full Text View journal details
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