Home LiteratureArticle Details
PMID: 1706521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcript elongation and termination are competitive kinetic processes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 6 ·1991-03-15 ·Pages 2307-11

von Hippel PH, Yager TD

Abstract

In this paper, we develop a kinetic approach to predict the efficiency of termination at intrinsic (factor independent) terminators of Escherichia coli and related organisms. In general, our predictions agree well with experimental results. Our analysis also suggests that termination efficiency can readily be modulated by protein factors and environmental variables that shift the kinetic competition toward either elongation or termination. A quantitative framework for the consideration of such regulatory effects is developed and the strengths and limitations of the approach are discussed.

MeSH Terms
DNA, Bacterial/genetics DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Kinetics Models, Genetic RNA, Bacterial/genetics Terminator Regions, Genetic Thermodynamics Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Bacterial DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
von Hippel P H
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229.
Yager T D
References (20)
20 references, click to expand
  1. Thermodynamic analysis of the transcription cycle in E. coli.
    Biophys Chem. 1990 Aug 31;37(1-3):239-50 PMID: 1704801
  2. Ribonucleic acid chain elongation by Escherichia coli ribonucleic acid polymerase. I. Isolation of ternary complexes and the kinetics of elongation.
    J Biol Chem. 1974 Oct 25;249(20):6675-83 PMID: 4608711
  3. Transcription termination factor rho mediates simultaneous release of RNA transcripts and DNA template from complexes with Escherichia coli RNA polymerase.
    J Biol Chem. 1985 May 10;260(9):5826-31 PMID: 3886662
  4. Contributions of RNA secondary structure and length of the thymidine tract to transcription termination at the thr operon attenuator.
    J Biol Chem. 1988 Jan 5;263(1):472-9 PMID: 2961747
  5. Improved free-energy parameters for predictions of RNA duplex stability.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9373-7 PMID: 2432595
  6. Identification and characterization of mutants affecting transcription termination at the threonine operon attenuator.
    J Mol Biol. 1985 Jun 25;183(4):529-41 PMID: 2410621
  7. Predicting DNA duplex stability from the base sequence.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3746-50 PMID: 3459152
  8. RNA chain elongation by Escherichia coli RNA polymerase. Factors affecting the stability of elongating ternary complexes.
    J Mol Biol. 1990 May 5;213(1):79-108 PMID: 1692594
  9. Genetic analysis of the tryptophan operon regulatory region using site-directed mutagenesis.
    J Mol Biol. 1984 May 25;175(3):299-312 PMID: 6374159
  10. Specificity of release by Escherichia coli transcription termination factor rho of nascent mRNA transcripts initiated at the lambda PR.
    J Biol Chem. 1984 Jul 10;259(13):8664-71 PMID: 6330119
  11. Transcription termination at the tryptophan operon attenuator is decreased in vitro by an oligomer complementary to a segment of the leader transcript.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2181-5 PMID: 6179092
  12. Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter.
    Biochemistry. 1980 Jul 8;19(14):3245-53 PMID: 6996702
  13. Escherichia coli tryptophan operon leader mutations, which relieve transcription termination, are cis-dominant to trp leader mutations, which increase transcription termination.
    J Mol Biol. 1980 Sep 5;142(1):123-9 PMID: 6159477
  14. Rho-independent termination: dyad symmetry in DNA causes RNA polymerase to pause during transcription in vitro.
    Nucleic Acids Res. 1981 Feb 11;9(3):563-77 PMID: 7012794
  15. Synthetic sites for transcription termination and a functional comparison with tryptophan operon termination sites in vitro.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4180-4 PMID: 7027254
  16. Nucleotide sequence of trpE of Salmonella typhimurium and its homology with the corresponding sequence of Escherichia coli.
    J Mol Biol. 1982 Mar 5;155(3):235-46 PMID: 7042989
  17. Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4833-7 PMID: 368800
  18. The regulatory region of the trp operon of Serratia marcescens.
    Nature. 1978 Dec 14;276(5689):684-9 PMID: 366432
  19. The attenuator of the tryptophan operon of Escherichia coli. Heterogeneous 3'-OH termini in vivo and deletion mapping of functions.
    J Mol Biol. 1977 Nov 25;117(1):227-47 PMID: 340702
  20. RNA 3'-end formation.
    Genes Dev. 1989 Dec;3(12B):2218-22 PMID: 2628168
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.6.2307
Published
1991-03-15
Pages
2307-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51220
Subset
IM
Grants
NIGMS NIH HHS · GM-15792 · United States
NIGMS NIH HHS · GM-29158 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.6.2307 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2