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PMID: 1321032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 'second-codon rule' and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes.

The EMBO journal ·Vol. 11 ·No. 7 ·1992-07-00 ·Pages 2433-46

Nishizawa M, Okazaki K, Furuno N, Watanabe N, Sagata N

Abstract

The c-mos proto-oncogene product, Mos, functions in both early (germinal vesicle breakdown) and late (metaphase II arrest) steps during meiotic maturation in Xenopus oocytes. In the early step, Mos is only partially phosphorylated and metabolically unstable, while in the late step it is fully phosphorylated and highly stable. Using a number of Mos mutants expressed in oocytes, we show here that the instability of Mos in the early step is determined primarily by its penultimate N-terminal residue, or by a rule referred to here as the 'second-codon rule'. We demonstrate that unstable Mos is degraded by the ubiquitin-dependent pathway. In the late step, on the other hand, Mos is stabilized by autophosphorylation at Ser3, which probably acts to prevent the N-terminus of Mos from being recognized by a ubiquitin-protein ligase. Moreover, we show that Ser3 phosphorylation is essential for Mos to exert its full cytostatic factor (CSF) activity in fully mature oocytes. Thus, a few N-terminal amino acids are primary determinants of both the metabolic stability and physiological activity of Mos during the meiotic cell cycle.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Codon DNA Electrophoresis, Polyacrylamide Gel Humans Meiosis Molecular Sequence Data Ovum/cytology Peptide Mapping Phosphorylation Phosphotransferases/metabolism Precipitin Tests Protein-Tyrosine Kinases/genetics,metabolism Proto-Oncogene Mas Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-mos Serine/metabolism Ubiquitins/metabolism Xenopus
Chemicals
Codon MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins Ubiquitins Serine DNA Phosphotransferases Protein-Tyrosine Kinases Proto-Oncogene Proteins c-mos
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nishizawa M
Division of Molecular Genetics, Kurume University, Fukuoka, Japan.
Okazaki K
Furuno N
Watanabe N
Sagata N
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-07-00
Pages
2433-46
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556718
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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