Abstract
The substrates of ubiquitin-dependent proteolytic pathways include both damaged or otherwise abnormal proteins and undamaged proteins that are naturally short-lived. Few specific examples of the latter class have been identified, however. Previous work has shown that the cell type-specific MAT alpha 2 repressor of the yeast Saccharomyces cerevisiae is an extremely short-lived protein. We now demonstrate that alpha 2 is conjugated to ubiquitin in vivo. More than one lysine residue of alpha 2 can be joined to ubiquitin, and some of the ubiquitin moieties form a Lys48-linked multiubiquitin chain. Overexpression of degradation-impaired ubiquitin variants was used to show that at least a significant fraction of alpha 2 degradation is dependent on its ubiquitination.
MeSH Terms
Amino Acid Sequence
Fungal Proteins/genetics,isolation & purification,metabolism
Genes, Fungal
Genes, myc
Homeodomain Proteins
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Plasmids
Protein Processing, Post-Translational
Repressor Proteins/genetics,isolation & purification,metabolism
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Ubiquitins/metabolism
Chemicals
Fungal Proteins
Homeodomain Proteins
MATA2 protein, S cerevisiae
Repressor Proteins
Saccharomyces cerevisiae Proteins
Ubiquitins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hochstrasser M
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
Ellison M J
Chau V
Varshavsky A
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