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PMID: 1320256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity.

Nucleic acids research ·Vol. 20 ·No. 12 ·1992-06-25 ·Pages 3159-65

Morimyo M, Hongo E, Hama-Inaba H, Machida I

Abstract

A new gene mvrC conferring resistance to methyl viologen, a powerful superoxide radical propagator, was cloned on 13.5 kilo base (kb) EcoRI DNA fragment. It gave resistance against methyl viologen to even a wild-type strain with gene dosage dependence. From the physical maps obtained by restriction enzyme digestions, it was predicted to locate at 580 kbp (12.3 min) on the physical map of E.coli. This was confirmed by the Southern hybridization of lambda phages covering this region with mvrC probe. The DNA sequence of mvrC gene was determined and its deduced protein encoding a 12 kd hydrophobic protein was confirmed by maxicell labeling of MvrC protein.

MeSH Terms
Amino Acid Sequence Antiporters Bacterial Proteins/chemistry,genetics Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements/genetics Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Escherichia coli Proteins Genes, Bacterial Membrane Proteins/chemistry,genetics Molecular Sequence Data Paraquat/pharmacology Plasmids/genetics Restriction Mapping
Chemicals
Antiporters Bacterial Proteins DNA Transposable Elements Escherichia coli Proteins Membrane Proteins EmrE protein, E coli Paraquat
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morimyo M
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Hongo E
Hama-Inaba H
Machida I
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.12.3159
Published
1992-06-25
Pages
3159-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312453
Subset
IM
Databases
GENBANK
M62732
External Links
PubMed source DOI: 10.1093/nar/20.12.3159 PMC Full Text View journal details
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