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PMID: 1317074 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simian virus 40 mutants with amino-acid substitutions near the amino terminus of large T antigen.

Virus genes ·Vol. 6 ·No. 2 ·1992-04-00 ·Pages 107-18

Peden KW, Pipas JM

Abstract

A series of amino-acid substitution mutants has been made with changes in the region of simian virus 40 large tumor antigen (T antigen) that is shared with the small tumor antigen (t antigen). Both single and multiple amino-acid replacements were obtained using the heteroduplex deletion loop method and sodium bisulfite as the mutagen. The mutants could be divided into five phenotypic classes on the basis of their biological properties: a) mutants whose changes did not affect their ability to propagate on permissive monkey cells, nor to transform nonpermissive rodent cells; b) mutants that were not viable, replicated their DNA to 5% or less of wild type, but were positive for transformation; c) mutants that were not viable, replicated their DNA to 5% or less of wild type, and were defective for transformation; and d) mutants that completely lost all three activities coordinately. In addition, one mutant with changes in this region, 5002, replicated its DNA to about 50% of wild type, had an impaired transformation activity, and produced virions at a level of about 4% that of wild type.

MeSH Terms
Amino Acid Sequence Animals Antigens, Polyomavirus Transforming/genetics Base Sequence Cells, Cultured DNA Replication DNA, Viral/biosynthesis Fluorescent Antibody Technique Genetic Complementation Test Genetic Markers Molecular Sequence Data Mutation Simian virus 40/genetics,growth & development Transfection Transformation, Genetic Viral Plaque Assay
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peden K W
Howard Hughes Medical Institute Laboratory, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD.
Pipas J M
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Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1007/BF01703060
Published
1992-04-00
Pages
107-18
Language
English
Region
United States
NLM ID
8803967
Subset
IM
Grants
NCI NIH HHS · 5 PO1 CA16519 · United States
NCI NIH HHS · CA40586 · United States
External Links
PubMed source DOI: 10.1007/BF01703060 View journal details
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