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PMID: 1314807 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The lemA gene required for pathogenicity of Pseudomonas syringae pv. syringae on bean is a member of a family of two-component regulators.

Journal of bacteriology ·Vol. 174 ·No. 9 ·1992-05-00 ·Pages 3011-20

Hrabak EM, Willis DK

Abstract

The lemA gene of the plant pathogen Pseudomonas syringae pv. syringae is required for disease lesion formation on bean plants. Cosmid clones that complemented a lemA mutant in trans were isolated previously. The lemA gene was localized by subcloning and transposon mutagenesis. The lemA region and flanking DNA were sequenced, and an open reading frame of 2.7 kb was identified. The nucleotide and predicted amino acid sequences of the lemA gene showed sequence similarity to a family of prokaryotic two-component regulatory proteins. Unlike most of the previously described two-component systems, the lemA gene product contained homology to both components in one protein. Mutations introduced upstream and downstream of the lemA gene failed to locate a gene for a second protein component but identified the putative cysM gene of P. syringae pv. syringae. The cysM gene was located upstream of the lemA gene and was divergently transcribed. The lemA gene product was expressed at low levels in P. syringae pv. syringae and appeared to be positively auto-regulated.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA Transposable Elements Gene Expression Regulation, Bacterial Histidine Kinase Molecular Sequence Data Multigene Family/genetics Mutagenesis, Insertional Phosphorylation Protein Kinases/genetics Protein Processing, Post-Translational Pseudomonas/genetics Sequence Homology, Nucleic Acid Transcription Factors/genetics
Chemicals
Bacterial Proteins DNA Transposable Elements Transcription Factors Protein Kinases Histidine Kinase lemA protein, bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hrabak E M
Department of Plant Pathology, University of Wisconsin, Madison 53706.
Willis D K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.9.3011-3020.1992
Published
1992-05-00
Pages
3011-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205956
Subset
IM
Grants
NIGMS NIH HHS · GM07215 · United States
Databases
GENBANK
M79367, M79368, M79369, M79370, M79371, M79372, M80477, M87491, M87492, S97385
External Links
PubMed source DOI: 10.1128/jb.174.9.3011-3020.1992 PMC Full Text View journal details
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