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PMID: 2111016 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Autogenous regulation of the Bordetella pertussis bvgABC operon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 10 ·1990-05-00 ·Pages 3763-7

Roy CR, Miller JF, Falkow S

Abstract

The bvgABC operon of the bacterial pathogen Bordetella pertussis encodes a sensory transduction system that regulates the expression of several virulence genes in response to environmental stimuli. In this study we have examined the transcriptional regulation of the bvgABC operon. Transcriptional bvg::lacZYA fusions in Escherichia coli show that the bvgABC operon is autogenously activated. Autoactivation is inhibited by the same environmental stimuli that result in the lack of expression of bvg-activated genes in B. pertussis. These observations were confirmed in B. pertussis using a chromosomal chloramphenicol acetyltransferase transcriptional fusion in bvgC. Transcriptional initiation sites upstream of bvgA were mapped by primer extension analysis in E. coli and B. pertussis. Two differentially regulated bvg promoters were identified. The bvgP1 promoter is a positively autoregulated promoter located 90 base pairs upstream of bvgA. The bvgP2 promoter is located 141 base pairs upstream of bvgA and does not appear to require any positive regulatory factors for activity. These results suggest a model describing the regulatory events that take place upstream of the bvgABC operon.

MeSH Terms
Base Sequence Bordetella pertussis/genetics,pathogenicity Cloning, Molecular Conjugation, Genetic DNA, Recombinant/metabolism Escherichia coli/genetics,growth & development Gene Expression Regulation, Bacterial Models, Genetic Molecular Sequence Data Oligonucleotide Probes Operon Plasmids Recombinant Fusion Proteins/metabolism Restriction Mapping Transcription, Genetic Virulence/genetics beta-Galactosidase/genetics,metabolism
Chemicals
DNA, Recombinant Oligonucleotide Probes Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roy C R
Department of Microbiology and Immunology, Stanford University, CA 94305.
Miller J F
Falkow S
References (23)
23 references, click to expand
  1. A SIMPLIFIED LIQUID CULTURE MEDIUM FOR THE GROWTH OF HEMOPHILUS PERTUSSIS.
    J Bacteriol. 1949 Aug;58(2):127-34 PMID: 16561764
  2. Antigenic modulation of Bordetella pertussis.
    J Hyg (Lond). 1960 Mar;58:57-93 PMID: 14413273
  3. Environmental regulation of expression of virulence determinants in Bordetella pertussis.
    J Bacteriol. 1989 Nov;171(11):6206-12 PMID: 2478524
  4. Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7850-4 PMID: 3020561
  5. The bvgA gene of Bordetella pertussis encodes a transcriptional activator required for coordinate regulation of several virulence genes.
    J Bacteriol. 1989 Nov;171(11):6338-44 PMID: 2553677
  6. Improved single and multicopy lac-based cloning vectors for protein and operon fusions.
    Gene. 1987;53(1):85-96 PMID: 3596251
  7. High efficiency transformation of E. coli by high voltage electroporation.
    Nucleic Acids Res. 1988 Jul 11;16(13):6127-45 PMID: 3041370
  8. Analysis of Bordetella pertussis virulence gene regulation by use of transcriptional fusions in Escherichia coli.
    J Bacteriol. 1989 Nov;171(11):6345-8 PMID: 2553678
  9. Sequences required for expression of Bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6671-5 PMID: 2549542
  10. Coordinate regulation and sensory transduction in the control of bacterial virulence.
    Science. 1989 Feb 17;243(4893):916-22 PMID: 2537530
  11. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  12. Genetic analysis of a region of the Bordetella pertussis chromosome encoding filamentous hemagglutinin and the pleiotropic regulatory locus vir.
    J Bacteriol. 1988 Jul;170(7):2904-13 PMID: 2898470
  13. Phase variation in Bordetella pertussis by frameshift mutation in a gene for a novel two-component system.
    Nature. 1989 Mar 16;338(6212):266-9 PMID: 2537932
  14. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  15. The construction of a cloning vector designed for gene replacement in Bordetella pertussis.
    Gene. 1986;50(1-3):133-40 PMID: 2884169
  16. Expression of glnA in Escherichia coli is regulated at tandem promoters.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1979-83 PMID: 2858855
  17. Extension inhibition analysis of translation initiation complexes.
    Methods Enzymol. 1988;164:419-25 PMID: 2468068
  18. Two trans-acting regulatory genes (vir and mod) control antigenic modulation in Bordetella pertussis.
    J Bacteriol. 1988 Nov;170(11):5059-66 PMID: 2903140
  19. Tn5-induced mutations affecting virulence factors of Bordetella pertussis.
    Infect Immun. 1983 Oct;42(1):33-41 PMID: 6311749
  20. Protein phosphorylation and regulation of adaptive responses in bacteria.
    Microbiol Rev. 1989 Dec;53(4):450-90 PMID: 2556636
  21. Genetic analysis of phase change in Bordetella pertussis.
    Infect Immun. 1984 Jan;43(1):263-9 PMID: 6317569
  22. Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):653-7 PMID: 6187001
  23. Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression.
    Plasmid. 1985 Mar;13(2):149-53 PMID: 2987994
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.10.3763
Published
1990-05-00
Pages
3763-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53983
Subset
IM
Grants
NIAID NIH HHS · AI23945 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.10.3763 PMC Full Text View journal details
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