Home LiteratureArticle Details
PMID: 2550761 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic and structural characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria.

Molecular & general genetics : MGG ·Vol. 218 ·No. 1 ·1989-07-00 ·Pages 127-36

Bonas U, Stall RE, Staskawicz B

Abstract

The avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria was cloned and found to be localized on a self-transmissable plasmid. Genetic analysis of an avrBs3 insertion mutation revealed that avrBs3 constitutes a single locus, specifying the resistant phenotype on pepper plants. Southern blot experiments showed that no DNA sequences homologous to avrBs3 were present in other races of X. c. pv. vesicatoria, which are unable to induce a hypersensitive reaction on ECW-30R. However, the DNA of several different pathovars of X. campestris hybridized to the avrBs3 probe. A deletion analysis defined a region of 3.6-3.7 kb essential for avrBs3 activity. The nucleotide sequence of this region was determined. A 3561 nucleotide open reading frame (ORF1), encoding a 125,000 dalton protein, was found in the 3.7 kb region that was sufficient for avrBs3 activity. A second long ORF (2351 nucleotides) was identified on the other strand. A remarkable feature of both ORFs is the presence of 17 direct repeats of 102 bp which share 91%-100% homology with each other.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data Mutation Phenotype Plant Diseases Plasmids Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic Xanthomonas/genetics
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bonas U
Department of Plant Pathology, University of California, Berkeley 94720.
Stall R E
Staskawicz B
References (28)
28 references, click to expand
  1. Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6743-7 PMID: 16593982
  2. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  3. Codon usage tabulated from the GenBank Genetic Sequence Data.
    Nucleic Acids Res. 1988;16 Suppl:r315-402 PMID: 3368329
  4. Characterization and expression of two avirulence genes cloned from Pseudomonas syringae pv. glycinea.
    J Bacteriol. 1988 Oct;170(10):4846-54 PMID: 3049552
  5. Erythrocyte spectrin is comprised of many homologous triple helical segments.
    Nature. 1984 Sep 13-19;311(5982):177-80 PMID: 6472478
  6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  7. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  8. The avirulence gene avrBs1 from Xanthomonas campestris pv. vesicatoria encodes a 50-kD protein.
    Mol Plant Microbe Interact. 1988 May-Jun;1(5):191-8 PMID: 2979910
  9. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
    EMBO J. 1985 Apr;4(4):891-8 PMID: 2990912
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):6024-8 PMID: 16593517
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  13. An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin.
    EMBO J. 1985 Sep;4(9):2145-51 PMID: 15938047
  14. Hypersensitivity as the primary event in resistance to fungal parasites.
    Nature. 1974 May 10;249(453):186-7 PMID: 4833528
  15. Molecular characterization and nucleic acid sequence of an avirulence gene from race 6 of Pseudomonas syringae pv. glycinea.
    J Bacteriol. 1987 Feb;169(2):572-8 PMID: 3027035
  16. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  17. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  18. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  19. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  20. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  21. Prediction of protein conformation.
    Biochemistry. 1974 Jan 15;13(2):222-45 PMID: 4358940
  22. Production of single-stranded plasmid DNA.
    Methods Enzymol. 1987;153:3-11 PMID: 3323803
  23. Large microtubule-associated protein of T. brucei has tandemly repeated, near-identical sequences.
    Science. 1988 Jul 22;241(4864):459-62 PMID: 3393912
  24. Gene-for-gene interactions of five cloned avirulence genes from Xanthomonas campestris pv. malvacearum with specific resistance genes in cotton.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6415-9 PMID: 16593751
  25. The repeat domain of Escherichia coli haemolysin (HlyA) is responsible for its Ca2+-dependent binding to erythrocytes.
    Mol Gen Genet. 1988 Nov;214(3):553-61 PMID: 3063951
  26. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  27. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea.
    J Bacteriol. 1987 Dec;169(12):5789-94 PMID: 2824447
  28. Shotgun DNA sequencing using cloned DNase I-generated fragments.
    Nucleic Acids Res. 1981 Jul 10;9(13):3015-27 PMID: 6269069
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330575
Published
1989-07-00
Pages
127-36
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330575
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2